乙肝孕妇非器官特异性自身抗体的检测与分析

目的了解乙肝孕妇血清中非器官特异性自身抗体存在状况，探讨孕妇乙型肝炎病毒（HBV）感染与机体自身免疫的关系。方法选用Hep-2细胞、大鼠胃、大鼠。肾为基质制成生物薄片作为抗原，采用生物薄片的马赛克技术（Biochipmosaic technology）应用间接免疫荧光法（IIF）对168例HBV孕妇和44例健康孕妇的血清行抗核抗体（ANA）、抗线粒体抗体（AMA）、抗平滑肌抗体（ASMA）检测，同时采用免疫印迹法（VCB）、速率散射法、金标法分别对受检者的血清进行可提取核抗原抗体谱（ENA）、类风湿因子（RF），抗-dsDNA检测。结果168例HBV孕妇检出多种非器官特异性自身抗体，总阳性率为19．4％，显著高于健康孕妇对照组（P〈0．05），检出的自身抗体以低滴度为主且以ANA，RF为多见。自身抗体阳性组与自身抗体阴性组孕妇的年龄、孕周及ALT水平无明显差异（P〉0．05），而阳性组HBeAg的检出率显著高于阴性组（P〈0．01）。结论孕妇HBV感染可引发机体自身免疫反应，产生多种以低滴度为主的非器官特异性自身抗体，这种免疫反应与HBV复制水平有关，而与肝脏功能的损害程度无关。

Detection of Non - organ - specific Autoantibodies in Pregnant Women With HBV Infection

Objective To investigate non-organ-specific autoantibodies in pregnant women with HBV infection and to explore the relationship between HBV infection and presence of autoantibodies. Methods Hep-2 cells,rat renal tissue and rat stomach tissue were used as substrates to prepare biochips for ANA, AMA and ASMA detection by indirect immunofluorescence assay in the serum samples from 168 HBV infected pregnant women and 44 healthy pregnant women served as control. ENA,RF,anti-dsDNA were measured by western blot(WB),turbidimetric assay and colloidal gold respectively. Results The positive rate of non-organ-specific autoantibodies in HBV infected pregnant women was significantly higher as compared with the healthy pregnant women (31.55% vs 13.64%, P<0.05). Low titer of autoantibodies were presented dominantly among the HBV pregnant women,and most of them were ANA and RF. There were no remarkable differences in the age, gestation weeks and ALT levels between the autoantibodies positive and negative groups (P>0.05), and the autoantibody positive rate in the HBV infected pregnant women with HBeAg was remarkably higher than those without( P<0.01). Conclusions HBV infection in pregnant women can induce autoimmune reactions resulting in production of non-organ-specific autoantibodies.The autoimmune reaction has no connection with the severity of liver dysfunction, but links with replicating level of HBV.