小蘖碱对高脂血症鼠脂联素的调控作用及其对血脂指标和斑块形成的影响

目的 研究小蘖碱对高脂血症鼠脂联素的调控作用及其对血脂指标和斑块形成的影响。 方法 在40只Wistar雄性鼠随机选取30只并喂养高脂饲料饮食进行高血脂(HLP)造模,对照组(n=10)则喂养正常食物并自由摄取水。10周后,将HLP模型随机分为3组: HLP组(n=10)、HLP+LBB组(n=10)和HLP+HBB组(n=10)。对HLP+LBB组鼠进行低剂量小檗碱(berberine, BB)灌胃(150 mg/(kg·d)), HLP+HBB组高剂量小檗碱灌胃(300 mg/(kg·d)),对照组和HLP组则以生理盐水代替。连续喂养10周后测定并比较4组鼠血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)、血清总脂联素和高分子量脂联素含量,以及心肌梗死斑块面积。 结果 造模后,与对照组相比,HLP组大鼠TG、TC和LDL-C显著升高,HDL-C水平显著下降(P<0.05)。提示HLP大鼠造模成功。与HLP组相比,HLP+LBB组、HLP+HBB组TG、TC和LDL-C显著降低(P<0.001),HLP+LBB组HDL-C水平显著升高(P<0.05)。四组大鼠血清总脂联素含量差异无统计学意义(P>0.05),但HLP组中高分子量脂联素与总脂联素的比值显著低于对照组, 而HLP+HBB组显著高于HLP组(P<0.05)。与对照组比较,AdipoR1在HLP组中表达下调(P<0.05), 而HLP+HBB组显著高于HLP组,差异有统计学意义(P<0.05)。AdipoR2在HLP组中的表达无明显变化,但HLP+HBB组显著高于对照组(P<0.05)。HLP+HBB组梗塞面积(11.4±1.0)%)]显著低于HLP组(52.2±0.9)%]和HLP+LBB组(33.1±1.1)%](均P<0.05)。 结论 小蘖碱可对抗高脂血症鼠血中脂联素的下调,这一干预可能在血脂指标和斑块形成方面起到改善作用。

Role of berberine in regulating adiponectin and its impact on blood lipidindexes and atheromatous plaque formation in hyperlipidemia rats

Objective To explore the role of berberine in regulating adiponectin and its influence on blood lipid indexes and atheromatous plaque formation in rats with hyperlipidemia(HLP). Methods Hyperlipidemia rat models were induced by feeding male Wistar rats with high-cholesterol diet for 30 days, and another 10 rats treated with regular food and water were selected as the control group. After 10 weeks, the 30 hyperlipidemia rats were randomly assigned into 3 groups, HLP group (n=10), HLP+LBB group (n=10) and HLP+HBB group (n=10). The latter two groups were respectively given low-dosage (150 mg/(kg·d)) and high-dosage (300 mg/(kg·d)) berberine for 10 weeks, while the control group and the HLP group were given normal saline. After 10 weeks, the serum levels of triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), serum total adiponetin and high molecular weight (HMW) adiponetin were tested and compared between the four groups; meanwhile, the infarction areas of the rat hearts were evaluated and compared. Results Compared with the control group, the serum levels of TG, TC and LDL-C in the HLP group were significantly increased, but the serum level of HDL-C was significantly decreased, suggesting that the HLP rat models were successfully established. Compared with the HLP group, the serum levels of TG, TC and LDL-C in the HLP+LBB group and the HLP+HBB group were significantly decreased (P<0.001), but the serum level of HDL-C in the HLP+LBB group was significantly increased (P<0.05). No statistically significant difference was found in the serum total adiponectin level among the 4 groups (P>0.05). The ratio of HMW adiponetin to total adiponetin was significantly lower in the HLP group than in the control group,but it was significantly higher in the HLP+HBB group than in the HLP group (P<0.05). Compared with the control group, the expression of AdipoR1 protein in the HLP group was down-regulated, but it was significantly higher in the HLP+HBB group than in the HLP group, with a statistically significant difference (P<0.05). The expression of AdipoR2 protein in the HLP group showed no apparent variation, but it was significantly higher in the HLP+HBB group than in the control group(P<0.05). The infarction area in the HLP+HBB group was significantly less than those in the HLP group and the HLP+LBB group ((11.4±1.0)% vs.(52.2±0.9)%, (11.4±1.0)% vs. (33.1±1.1)%, both P<0.05). Conclusions Berberine can confront the deregulation of adiponectin expression in hyperlipidemia rats, and it may be a potential therapeutic strategy for blood lipid and atheromatous plaque formation.