河南省2010年肠道病毒71型VP1基因特征分析

目的对河南省2010年手足口病监测标本进行病原分离及VP1基因测序,了解分离病毒的基因特征及分子流行病学特点。方法将2010年收集的手足口病患者粪便标本和肛拭子标本840份进行病毒分离鉴定并对34株病毒分离株测定肠道病毒71型VP1全序,利用生物信息学软件对序列分析,构建序列系统进化树。结果测序获得34株来自河南省11个地市的VP1全长序列,分离株间的VP1区核苷酸相似性为96.3%～100%,系统进化分析显示属于C4基因型的C4a亚群,所有分离株均处于同一进化分支,轻重症间无明显差别。在2003~2009年的河南省和其他7省亦发现有C4a亚群存在。结论 2010年河南EV71分离株为C4基因型的C4a亚群,河南省2008年以来的分离株与2004年以来的中国大陆优势株流行趋势完全一致。

Analysis on genomic characteristics of enterovirus 71 types of VP1 gene in Henan of 2010

OBJECTIVE To reveal the genomic characteristics of enterovirus 71 strains of VP1 gene from Hand-Foot-Mouth Disease,and analyze their molecular epidemiology.METHODS A total of 840 clinical specimens were collected and isolated,and 34 enterovirus among 71 types of VP1 gene were analyzed by bioinformatics software.RESULTS The VP1 of 34 isolated specimens was gained by sequencing.The nucleotide similarities of VP1 gene were at the range of 96.3% to 100%.Phylogenetic analysis based on VP1 regions revealed that the Henan enterovirus 71 in 2010 belonged to C4a in subgenotype C4,and no difference was found between the severe and mild illness.Cluster C4a enterovirus 71 was also found in henan and other seven provinces during 2003-2009.CONCLUSION 71 isolated strains of enterovirus in Henan of 2008 belong to C4a in the C4 subgenotype,which is in line with the predominant virus circulating in mainland China since 2004.