Keap1 rs11668429和AHSA2 rs728825与延边地区原发性肝癌发病风险的研究

目的 探讨Keap1 rs11668429和AHSA2 rs728825与延边地区肝癌易感性的关系。方法 选取延边地区192名肝癌患者为病例组、190名健康人为对照组进行研究。采用PCR - RFLP方法检测基因型及基因频率。采用二元logistic回归模型分析Keap1 rs11668429位点和AHSA2 rs728825位点的单核苷酸多态性与肝癌易感性的关系。结果 Keap1 rs11668429位点有TT、TG、GG三种基因型；AHSA2 rs728825位点有GG、GA、AA三种基因型。在Keap1 rs11668429位点中，携带TT基因型的个体比携带TG、GG基因型的个体患肝癌的风险更大（OR = 1.681，95%CI:1.068～2.644,P = 0.025）；在AHSA2 rs728825位点中携带GG基因型的个体比携带GA、AA基因型个体更易患肝癌(OR = 2.275,95%CI:1.469～3.523,P＜0.01)。分析两个位点的基因多态性对肝癌易感性的联合影响，结果表明病例组和对照组的联合基因频率分布差异有统计学意义(P＜0.01)。携带一个危险基因型和携带两个危险基因型的个体比不携带危险基因型的个体更易患肝癌(OR = 3.046，95%CI:1.705～5.442，P＜0.01)。结论 Keap1 rs11668429(T＞G)和AHSA2 rs728825(G＞A)位点多态性与延边地区肝癌易感性有明显相关性。

Keap1 rs11668429 and AHSA2 rs728825 and the risk of primary liver cancer in Yanbian region

Objective To investigate the relationship between Keap1 rs11668429 and AHSA2 rs728825 and susceptibility to liver cancer in Yanbian region. Methods A total of 192 liver cancer patients in Yanbian region were selected as the case group and 190 healthy individuals as the control group for the study. The genotype and gene frequency were detected by PCR-RFLP method. A binary logistic regression model was used to analyze the relationship between single nucleotide polymorphisms at the Keap1 rs11668429 locus and AHSA2 rs728825 locus and susceptibility to hepatocellular carcinoma. Results Keap1 rs11668429 locus had three genotypes, TT, TG and GG; AHSA2 rs728825 locus had three genotypes, GG, GA and AA. In Keap1 rs11668429 locus, individuals carrying TT genotype had a greater risk of liver cancer than those carrying TG and GG genotypes (OR=1.681, 95%CI: 1.068-2.644, P=0.025); at AHSA2 rs728825 locus, individuals carrying GG genotype were more likely to develop liver cancer than those carrying GA and AA genotypes (OR=2.275, 95%CI: 1.469-3.523, P<0.01). Analysis of the combined effect of gene polymorphisms at both loci on susceptibility to hepatocellular carcinoma showed statistically significant differences in the distribution of combined gene frequencies between the case and control groups (P<0.01). Individuals carrying one risk genotype and those carrying two risk genotypes were more likely to develop hepatocellular carcinoma than those not carrying the risk genotype (OR=3.046, 95%CI: 1.705-5.442, P<0.01). Conclusion Keap1 rs11668429 (T>G) and AHSA2 rs728825 (G>A) locus polymorphisms were significantly correlated with susceptibility to hepatocellular carcinoma in Yanbian region.