JNK1/2信号通路在砷致细胞凋亡中双向效应的Meta分析

目的 通过Meta分析探讨在砷在诱导细胞凋亡过程中JNK1/2信号通路发挥的作用及机制。 方法 在CNKI、维普、万方、SinoMed、PubMed、Web of Science、Cochrane、Spinger等数据库中检索文献。采用STAIR清单对文献质量进行评价，应用Stata 12.0和RevMan 5.3软件提取数据及分析；以标准化均数差(SMD)来描述其组间差别。 结果 本研究共纳入33篇文献。砷干预组Bax:SMD=9.41，95%CI(1.92，16.90)，凋亡细胞:SMD=10.46，95%CI(6.87，14.05)，Caspase-3:SMD=8.82，95%CI(0.79，16.85)，Cytochrome C:SMD=90.24，95%CI(34.45，146.03)，c-jun:SMD=81.66，95%CI(14.38，148.94)和P-JNK:SMD=139.06，95%CI(29.14，248.98)水平均高于对照组。Bcl-2:SMD=-3.78，95%CI(-7.17，-0.39)和PARP:SMD=-6.48，95%CI(-12.89，-0.07)的水平均低于对照组。亚组分析表明正常细胞中高剂量(＞5μmol)且长时间(≥24h)砷暴露可抑制JNK1和JNK2的蛋白表达，在癌细胞中低剂量(≤5μmol)且短时间(＜24h)砷暴露则会诱导JNK1和JNK2的表达。 结论 砷通过介导JNK1/2信号通路诱导细胞凋亡，且作用效果与细胞类型、作用时间和剂量有关。

Meta-analysis of bipartite effects of JNK1/2 signaling pathway in arsenic induced apoptosis

Abstract: Objective To investigate the role and mechanism of JNK1 /2 signaling pathway in inducing apoptosis. Methods Relevant studies were searched in databases such as CNKI，VIP，WanFang，SinoMed，PubMed，Web of Science，Cochrane，and Spinger. Stata12. 0 and RevMan5. 3 software were used to extract data for analysis. STAIR checklist was used to evaluate the quality of the literatures，and SMD was used to describe differences between groups. Results A total of 33 literatures were included in this study. The Bax: SMD = 9. 41，95% CI ( 1. 92，16. 90) ，apoptotic cell: SMD = 10. 46，95% CI ( 6. 87，14. 05) ，Caspase － 3: SMD = 8. 82，95% CI ( 0. 79，16. 85) ，Cytochrome C: SMD = 90. 24，95% CI ( 34. 45，146. 03) ，c －jun: SMD = 81. 66，95% CI ( 14. 38，148. 94) and P － JNK: SMD = 139. 06，95% CI ( 29. 14，248. 98) levels in the arsenic intervention group were higher than the control group. While the levels of Bcl － 2: SMD = － 3. 78，95% CI ( － 7. 17，－ 0. 39)and PARP: SMD = － 6. 48，95% CI ( － 12. 89，－ 0. 07) were lower than the control group. Subgroup analysis showed that high － dose ( ＞ 5μmol) and long － term ( ≥24h) arsenic exposure in normal cells could inhibit JNK1 and JNK2 protein expressions. Low － dose ( ≤5μmol) and short － term ( ＜ 24h) arsenic exposure in cancer cells could induce the expressions ofJNK1 and JNK2. Conclusion Arsenic induces apoptosis by mediating the JNK1 /2 signaling pathway，and the effect is related to cell type，duration of action，and dose.