米非司酮配伍米索前列醇对母胎界面孕酮诱导阻断因子及孕酮受体表达的影响

目的 研究米非司酮配伍米索前列醇对母胎界面孕酮诱导阻断因子（PIBF）及孕酮受体（PR）表达的影响. 方法 应用免疫组织化学染色法检测米非司酮药物流产成功（药流成功组,n =30）、失败（药流失败组,n=30）及正常早孕负压吸宫流产（手术组,n=30）绒毛及蜕膜组织中PIBF及PR的表达情况. 结果 PIBF表达于绒毛合体滋养层细胞、细胞滋养层细胞和蜕膜细胞胞质,PR表达于蜕膜组织细胞核.与手术组相比,药物流产的两组绒毛滋养层细胞中PIBF的表达差异无统计学意义,而在蜕膜细胞中,药物流产的两组PIBF、PR的表达明显下降,差异有统计学意义,且药流成功组PIBF及PR的表达低于药流失败组,差异有统计学意义. 结论 米非司酮配伍米索前列醇终止早期妊娠可能与PIBF及PR的表达降低有关,其成功率与PIBF、PR表达降低程度相关.

Expression of progesterone-induced blocking factors and progesterone receptors in the maternal-fetal interface treated with mifepristone and misoprostol

Objective To study the anti-early pregnancy mechanism of mifepristone and misoprostol by investiga- ting the expression of progesterone-induced blocking factor （PIBF） and progesterone receptor （PR） in the maternal-fetal interface of early pregnancy. Methods PIBF and PR were investigated with immunohistochemistry in 3 groups, inclu- cling group A （ successful medical abortion, n = 30）, group B （ failure medical abortion, n = 30） and group C （ induced a- bortion with vacuum aspiration, n = 30）. Results PIBF was mainly detected in the cytoplasm of syncytiotrophoblastie, cytotrophoblastic and decidual cells, and PR expressed on decidual cell nucleus. The expression of PIBF and PR in decidu- as of group A and B was lower than group C, with no difference of PIBF expression in villus. Both PIBF and PR had lower expression level in group A than in group B. Conclusion Mifepfistone may prevent pregnancy by lowering the expression of P1BF and PR in the maternal-fetal interface of early pregnancy, and the success rate of pregnancy was associated with the decline in their expression.