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HIV抗体免疫印迹法检测中不确定结果的分析研究
引用本文:谭卓,夏晓华,倪崖,李忠辉,周富英,孟哲峰,李坤,黄云坚.HIV抗体免疫印迹法检测中不确定结果的分析研究[J].国际流行病学传染病学杂志,2011,38(6):367-370.
作者姓名:谭卓  夏晓华  倪崖  李忠辉  周富英  孟哲峰  李坤  黄云坚
作者单位:1. 310018,杭州澳亚生物技术有限公司生物工程研究院
2. 浙江省临床检验中心,杭州,310014
3. 浙江省医学科学院,杭州,310013
4. 200032,复旦大学上海医学院医学分子病毒学重点实验室
基金项目:浙江省科技计划专项资金项目
摘    要:目的 收集HIV抗体免疫印迹法检测中出现的不确定结果,统计分析各种带型的临床意义,为检验人员提供判定结果的方法和建议.方法 采集187份不确定实验结果和随访复检结果,以随访最终结果为金标准,统计分析各种带型出现的频度和阳性预测值,分析非特异性条带出现的频度和复检结果.结果 条带gp160、p24的阳性预测值分别为32....

关 键 词:HIV抗体  免疫印迹法  不确定结果

Study on indeterminate results in western blot confirmatory testing for antibodies to HIV
TAN Zhuo,XIA Xiao-hua,NI Ya,LI Zhong-hui,ZHOU Fu-ying,MENG Zhe-feng,LI Kun,HUANG Yun-jian.Study on indeterminate results in western blot confirmatory testing for antibodies to HIV[J].International Journal of Epidemiology and Infectious Disease,2011,38(6):367-370.
Authors:TAN Zhuo  XIA Xiao-hua  NI Ya  LI Zhong-hui  ZHOU Fu-ying  MENG Zhe-feng  LI Kun  HUANG Yun-jian
Abstract:Objective To provide some methods on verifying indeterminate results in western blot confirmatory testing for HIV antibodies with statistic analysis.Methods 187 indeterminate samples with final results in follow-up visit were gathered and analyzed.Results Positive predictive value (PPV) of gp160 and p24 band were 32.14% and 18.84%,gp160/p24(both) were 100.00%.Other specific bands couldn't increase the PPV,and non-specific bands predictive negative result.Conclusions Technicians can verify the indeterminate results initially by discriminating the different bands.
Keywords:HIV antibodies  Western blot  Indeterminate results
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