血清GP73、AFP和AFP-L_3联合检测对原发性肝癌诊断的价值

目的探讨联合检测血清高尔基体蛋白73(GP73)、甲胎蛋白(AFP)和甲胎蛋白异质体(AFP-L3)对原发性肝癌(PHC)的诊断价值。方法采用酶联免疫吸附试验(ELISA)测定132例原发性肝癌(PHC组)、50例肝硬化和100例慢性乙型病毒性肝炎(良性肝病组)以及60名健康体检者(正常对照组)血清GP73含量,同时采用电化学发光法(ECL)检测血清AFP水平,采用AFP-L3亲和吸附离心管装置检测甲胎蛋白异质体百分比(AFP-L3%)。结果血清GP73、AFP和AFP-L3在PHC组的诊断敏感度分别为74.15%、59.22%和54.34%,在其他肝病组的特异性分别为81.45%、85.13%和97.24%。三者联合检测时,其诊断敏感度可提高至90.15%。结论血清GP73对PHC诊断具有较好的敏感度,AFP-L3具有较高的特异性。血清GP73、AFP和AFP-L3联合检测可有效提高PHC的临床诊断效果。

VALUE OF SERUM GP？3, AFP AND AFP- L3 JOINT DETECTION IN THE DIAGNOSIS OF PRIMARY HEPATIC CARCINOMA

Objective To explore the significance of serum golgi protein 73 （GP73） , alpha fetoprotein （AFP） and lectin -reactive alpha fetoprotein （AFP- L3 ） joint detection in the diagnosis of primary hepatic carcino- ma （PHC）. Methods ELISA, electrochemiluminescenee （ECL） and affinity adsorption centrifuge tube were used respectively to detect the serum level of GP73, AFP and the percent of AFP - L3 in 132cases of PHC, 150 cases of chronic hepatitis and liver cirrhosis, 60 cases of health people. Results The sensitivity of GP73, AFP and AFP - L3 in PHC was 74. 15% , 59. 22% and 54. 34%, the specificity of GP73, AFP and AFP - L3 in other hepatic disease group was 81.45%, 85.13% and 97.24% respectively. Joint detection could increase the sensitivity up to 90. 15%. Conclusion For diagnosis of PHC, GP73 is a high sensitivity mark while AFP - L3 is a high specificity mark. The joint detection of GP73, AFP and AFP - L3 can effectively increase the clinical diagnosis effect of PHC.