| 南宁市同期两起A（H3N2）流行性感冒疫情中流感病毒血凝素重链区基因特性 |
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| 引用本文： | 秦剑秋,刘海燕,林新勤,黄莉,范云燕.南宁市同期两起A（H3N2）流行性感冒疫情中流感病毒血凝素重链区基因特性[J].职业与健康,2011,27(6):611-613. |
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| 作者姓名： | 秦剑秋 刘海燕 林新勤 黄莉 范云燕 |
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| 作者单位： | 广西壮族自治区南宁市疾病预防控制中心微生物检验科,530011 |
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| 基金项目： | 广西南宁市科技局科技攻关项目 |
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| 摘 要： | 目的了解南宁市同一时期不同县区发生的两起A（H3N2）亚型流感疫情中流感病毒血凝素基因变异情况。方法从狗肾传代细胞中（MDCK）分离的A（H3N2）亚型流感病毒提取核糖核酸（RNA）,进行血凝素核苷酸全长序列测定,推导出其氨基酸序列,重点对血凝素重链区进行基因特性分析。结果两起疫情共分离到A（H3N2）亚型流感病毒5株,核苷酸和氨基酸序列分析结果表明,与2007年WHO推荐的北半球疫苗株A/Wisconsin/67/2005（H3）相比,核苷酸和氨基酸同源性分别为98.58%～98.89%和96.96%～97.87%,替换数分别为11～14个和7～10个,涉及3个重要的抗原位点。结论同期两起疫情分离到的A（H3N2）亚型流感病毒已发生抗原漂移且毒力有所改变,发生在不同县区的疫情所分离到的流感病毒性状不尽相同,同一起疫情中发病时间不同所分离出的流感病毒有一定基因差异。
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| 关 键 词： | 流感病毒 血凝素 基因 变异 |
Genetic Characteristics of HA1 Gene of Influenza A(H3N2) Virus Circulated in Simultaneous2 Outbreaks in Nanning City |
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| QIN Jian-qiu,LIU hai-yan,LIN Xin-qin,HUANG Li,FAN Yun-yan.Genetic Characteristics of HA1 Gene of Influenza A(H3N2) Virus Circulated in Simultaneous2 Outbreaks in Nanning City[J].Occupation and Health,2011,27(6):611-613. |
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| Authors: | QIN Jian-qiu LIU hai-yan LIN Xin-qin HUANG Li FAN Yun-yan |
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| Institution: | QIN Jian-qiu,LIU hai-yan,LIN Xin-qin,HUANG Li,FAN Yun-yan(Nanning Center for Disease Control and Prevention,Guangxi,530011,China) |
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| Abstract: | Objective]To understand the genetic characteristics of influenza A(H3N2) virus circulated in 2 outbreaks in the different area of Nanning at the same time. Methods]Viral RNA was extracted from the influenza A(H3N2) virus subtype isolated from MDCK,and was sequenced full-length hemagglutinin nucleotide,then the amino acid sequence was deduced.The analysis focused on the genetic characteristics of HA1 Gene. Results]A total of 5 strains of influenza A(H3N2) virus subtype were isolated.The results of nucleot... |
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| Keywords: | Influenza virus Hemagglutinin Gene Mutation |
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