Implantable vaccine development using in vitro antigen-pulsed macrophages absorbed on laser micro-structured Si scaffolds |
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| Affiliation: | 1. Department of Biology, University of Crete, Vassilika Vouton, Heraklion 71409, Crete, Greece;2. Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology, Vassilika Vouton, Heraklion 71409, Crete, Greece;3. Department of Physics, University of Crete, Vassilika Vouton, Heraklion 71409, Crete, Greece;1. Center for Immunobiology and Vaccine Development, Children''s Hospital Oakland Research Institute, Oakland, CA 94609, USA;2. Department of Molecular Genetics, University of Toronto, Toronto, ON, M55 1A8 Canada;1. Department of Epidemiology, Helmholtz Centre for Infection Research, Braunschweig, Germany;2. TWINCORE Centre for Experimental and Clinical Infection Research, Hannover, Germany;3. African Population and Health Research Center, Nairobi, Kenya;4. Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany;5. Hannover Medical School, Hannover, Germany;6. Department of Biometry, Epidemiology and Information Processing, University for Veterinary Medicine, Hanover, Germany;1. Centre for Epidemiology and Evidence, NSW Ministry of Health, North Sydney, NSW 2060, Australia;2. Centre for Epidemiology and Evidence, NSW Ministry of Health, Locked Mail Bag 961, North Sydney, NSW 2059 Australia;3. School of Public Health and Community Medicine, University of New South Wales, Sydney, NSW 2052, Australia;4. The Telethon Kids Institute, Subiaco, 6008 WA, Australia;5. Public Health Unit, South Eastern Sydney Local Health District, Randwick, NSW 2031, Australia;6. National Centre for Immunisation Research and Surveillance (NCIRS), Westmead, NSW 2145, Australia;7. Discipline of Paediatrics and Child Health, University of Sydney, NSW 2006, Australia;1. University Children''s Hospital Basel, Switzerland;2. University of Basel, Basel, Switzerland;1. Institution of Optics & Electronics, Chinese Academy of Sciences, Chengdu 610209, China;2. University of Chinese Academy of Sciences, Beijing 100039, China;3. School of Optoelectronic Information, University of Electronic Science and Technology of China, Chengdu 610054, China;1. The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China;2. Institute of Hematology, School of Medicine, Jinan University, Guangzhou, Guangdong, China |
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| Abstract: | To overcome the limiting antigenic repertoire of protein sub-units and the side effects of adjuvants applied in second generation vaccines, the present work combined in vitro and in vivo manipulations to develop biomaterials allowing natural antigen-loading and presentation in vitro and further activation of the immune response in vivo. 3-dimensional laser micro-textured implantable Si-scaffolds supported mouse macrophage adherence, allowed natural seeding with human serum albumin (antigen) and specific antibody and inflammatory cytokine production in vitro. Implantation of Si-scaffolds loaded with antigen-activated macrophages induced an inflammatory reaction along with antigen-specific antibody production in vivo, which could be detected even 30 days post implantation. Analysis of implant histology using scanning electron microscopy showed that Si-scaffolds could be stable for a 6-month period. Such technology leads to personalized implantable vaccines, opening novel areas of research and treatment. |
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| Keywords: | Silicon scaffold Laser manufacturing Immune response Macrophage Scaffold implantation Vaccination HSA" },{" #name" :" keyword" ," $" :{" id" :" kw0040" }," $$" :[{" #name" :" text" ," _" :" human serum albumin FCA" },{" #name" :" keyword" ," $" :{" id" :" kw0050" }," $$" :[{" #name" :" text" ," _" :" Freund complete adjuvant SEM" },{" #name" :" keyword" ," $" :{" id" :" kw0060" }," $$" :[{" #name" :" text" ," _" :" scanning electron microscopy WBC" },{" #name" :" keyword" ," $" :{" id" :" kw0070" }," $$" :[{" #name" :" text" ," _" :" white blood cells |
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