| 循环miRNAs分析检测方法研究进展 |
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| 引用本文： | 王晓军,周殿明. 循环miRNAs分析检测方法研究进展[J]. 环境与健康杂志, 2019, 36(1): 72-77 |
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| 作者姓名： | 王晓军 周殿明 |
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| 作者单位： | 天津市疾病预防控制中心卫生检测所,天津,300011;天津市疾病预防控制中心卫生检测所,天津,300011 |
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| 基金项目： | 天津市卫生计生委科技基金重点项目 |
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| 摘 要： | 循环miRNAs是重要的毒性标志分子,其含量极低、序列多样和长度较短的特性给分析检测带来挑战,成为决定其应用于新化学物质和材料的毒理学研究和临床应用的关键。该文综述了目前可用于循环miRNA分析检测的方法,包括传统微阵列法、测序法、RT-PCR法和生物传感方法,并对这些方法的技术特点和检测中存在的主要问题进行论述。
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| 关 键 词： | 循环miRNA 毒性标志物 微阵列法 测序法 RT-PCR法 生物传感 |
Advances on determination of circulating miRNA |
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| WANG Xiao-jun,ZHOU Dian-ming. Advances on determination of circulating miRNA[J]. Journal of Environment and Health, 2019, 36(1): 72-77 |
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| Authors: | WANG Xiao-jun ZHOU Dian-ming |
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| Affiliation: | (Institute for Hygienic Determination,Tianjin Centers for Disease Control and Prevention,Tianjin 300011,China) |
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| Abstract: | Circulating miRNA have been recently acknowledged as an important toxicity biomarkers.Challenge and difficulty for determination induced by sequence diversity,short fragment,affected the application of circulating miRNA in toxicologic study on new chemicals and materials,and clinical practice.In this paper,circulating miRNA detection techniques were reviewed as much as possible,such as traditional microarray,sequencing,RT-PCR and biological sensing,also the main factors and problem in the quantification. |
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| Keywords: | Circulating miRNAs Toxicity biomarker Microarray Sequencing RT-PCR Biological sensing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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