首页 | 本学科首页   官方微博 | 高级检索  
检索        

环境雌激素双酚A的生殖毒理研究
引用本文:邓茂先,吴德生,詹立.环境雌激素双酚A的生殖毒理研究[J].环境与健康杂志,2001,18(3):134-136.
作者姓名:邓茂先  吴德生  詹立
作者单位:1. 华西医科大学公共卫生学院环境卫生教研室
2. 成都市卫生防疫站
基金项目:国家自然科学基金重点项目 (30 0 30 1 2 0 )
摘    要:目的:探讨双酚A的雄性生殖毒理学机制。方法:通过口服染毒,采用组织化学和免疫组织化学分析双酚A对成年SD大鼠曲细精管细胞的形态结构,波形蛋白及p53表达的影响。结果:实验表明,两周染毒后0.5%双酚A引起生精细胞与支持细胞发生分离,排列紊乱,从基底膜脱落入管腔,支持细胞和生精细胞核染色质凝聚成絮状;生精细胞和间质细胞p53表达增强4倍;支持细胞波形蛋白消失。结论:双酚A引起的支持细胞和生精细胞分离是其生殖毒性的重要机制之一,这一病理过程可能与支持细胞的波形蛋白消失和支持细胞及生精细胞p53表达改变有关;间质细胞p53表达增强可能预示其潜在的抗雄激素毒性。

关 键 词:双酚A  生殖毒性  波形蛋白  p53基因表达  环境雌激素  环境污染物
文章编号:1001-5914(2001)03-0134-03
修稿时间:2000年9月11日

Study on Mechanism of Reproduction Toxicity of to Estrogic Bisphenol-A Related to Environment
DENG Mao-xian,WU De-sheng,ZHAN Li.Study on Mechanism of Reproduction Toxicity of to Estrogic Bisphenol-A Related to Environment[J].Journal of Environment and Health,2001,18(3):134-136.
Authors:DENG Mao-xian  WU De-sheng  ZHAN Li
Abstract:Objective To explore the mechanism of male reproductive toxicity of bisphenol A Methods The morphological characteristics of seminiferous tubule,vimentin filaments and p53 gene expression in sertoli's cells of adult SD rats orally exposed to 0.5% bisphenol A were observed and analyzed by histo chemical and immuno histochemical methods Results After 2 week exposure to 0.5% bisphenol A,the disattachment between sertoli's cell and spermatogonia,spermatogonia arranged in disorder and displacement of spermatogonia away from the basement membrance of seminiferous tubules as well as the flocculated chromatins of nuclei in sertoli's cells and spermatogonia were observed.Fourfold increase of p53 expression in nuclei of spermatogonia and leydig's cells and the collaps of vimentin filaments in sertoli's cells were also found. Conclusion The results suggested that the disattachment between sertoli's cells and spermatogonia,might be associated with the collaps of vimentin filaments in nuclei of sertoli's cells and the increase of p53 expression in nuclei of sertoli's cells and spermatogonia,which might be one of the important mechanisms of reproductive toxicity of bisphenol A.The increase of p53 expression in nuclei of leydig's cells might predict the potential anti androgenicity of bisphenol A
Keywords:Bisphenol  A  Reproductive toxicity  Vimentin filament  p53 expression
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号