血管内皮细胞生长因子基因多态性与病毒性脑膜炎易感性关系

目的探讨血管内皮细胞生长因子(VEGF)基因多态性与病毒性脑膜炎易感性关系。方法对138例病毒性脑炎患者和152例健康者进行研究。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法测定所有研究对象外周血样本的VEGF基因启动子区-460T/C、-116G/A和+405G/C位点的基因多态性,通过对病毒性脑炎组和对照组的基因频率的比较,对比分析。结果 +405G/C位点基因型频率(GG、GC、CC)在病例组为16.67%、46.38%、36.95%,对照组为28.95%、47.37%、23.68%,两者比较差异有统计学意义。病例组的G、C等位基因频率为39.86%、60.14%,对照组为52.63%、47.37%,两者差异也有统计学意义。而-460T/C、-116G/A位点病例组和对照组的基因型、等位基因频率比较差异均无统计学意义。结论病毒性脑膜炎的易感性可能与VEGF的+405G/C位点多态性有关,其中C基因可能是病毒性脑膜炎的易感基因,而G基因可能是保护基因。而-460T/C、-116G/A位点多态性与病毒性脑膜炎易感性无明确的关系。

Relationship of vascular endothelial growth factor gene polymorphisms and susceptibility to viral meningitis

Objective To explore the relationship between the genetic polymorphism vascular endothelial growth factor and susceptibility to viral meningitis.Methods A total of 138 cases of viral meningitis and 152 healthy subjects were studied.Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) method was adopt to determine VEGF gene promoter region of-460T/C,-116G/A,and +405G/C locus gene polymorphism for all specimens,comparing CETP VEGF gene polymorphism in viral meningitis group with control group,comparatively analysed.Results In +405 G/C sites the proportions of GG,GC,CC genotype in viral meningitis group were 16.67%,46.38%,36.95% respectively,and were 28.95%,47.37%,23.68% in the control group,the difference was statistically significant.The frequencies of G,C allele in viral meningitis group were 39.86%,60.14% respectively,and were 52.63%,47.37% in the control group,the difference was also statistically significant in comparison.But in-460T/C,-116G/A sites viral meningitis group and control groups of genotypes and allele frequency was not statistically significant in comparison.Conclusions VEGF +405G/C genetic polymorphism may be associated with the occurrence of viral meningitis.Where C allele may be the susceptive to gene viral meningitis,but G allele may be the protective gene.-460T/C,-116G/A genetic polymorphism has no definite relationship with the occurrence of viral meningitis.