诱导性大鼠慢性胰腺炎超微结构变化研究

目的观察Wistar大鼠胆源诱导性慢性胰腺炎超微结构变化。方法健康Wistar雄性大鼠50只,随机分为三组：空白对照组（10只）、实验对照组（10只）及模型组（30只）。空白对照组常规饲养。实验对照组暴露胆总管,模型组暴露并结扎胆总管。42d后取各组胰腺组织标本,醋酸双氧铀-柠檬酸铅双重染色,电镜观察组织变化。结果空白对照组胰腺腺泡细胞形态规整;实验对照组间质轻度水肿,线粒体、内织网正常,细胞核完好;模型组大鼠胰腺组织超微结构的改变：即胰腺星状细胞出现,胶原纤维的生成。结论慢性胆源性胰腺炎超微结构变化主要集中在胰腺的外分泌部及细胞间质。

Ultrastructure changes of induced-pancreatitis in rat

Objective To observe the pancreatic ultrastructural changes in rats with induced-pancreatitis in order to provide a theoretical basis for the early diagnosis of chronic pancreatitis.Methods 50 healthy Wistar rats were randomly divided into blank control group（10）,experimental control group（10） and model group（30）.Rats in each group were treated differently with routine breeding,exposed bile duct or exposed and ligated bile duct.42 days later,all pancreases were removed,stained and observed under electron microscopy.Results Pancreatic acinar cells were normal in the control group,while mild interstitial edema with normal mitochondria and weaving as well as intact nucleus were found in the experimental control group.However ultrastructural changes in pancreatic tissue of rats in model group showed emerging of pancreatic stellate cells and the formation of collagen fibers.Conclusions Electron microscopy can be used for the diagnosis and treatment evaluation of chronic pancreatitis.It is safe,simple and accurate and has great potential for early diagnosis of CP.