持续性次氯酸消毒对内镜终末漂洗水的消毒效果

 目的 探讨持续性次氯酸灌注对内镜终末漂洗水的消毒效果。方法 基线采集内镜终末漂洗水的水样,进行微生物培养和宏基因组二代测序鉴定。引入微酸性次氯酸水发生机后,在安装后的特定时间收集水样进行培养和宏基因组二代测序分析。结果 安装前共采集水样27份,其中10份合格,合格率为37.03%。不合格水样可检出放线根瘤菌、嗜麦芽窄食单胞菌、黄色微杆菌、贪铜菌、藤泽甲基杆菌、少动鞘氨醇单胞菌等水源性细菌;安装后共采集水样54份,均为合格。宏基因组二代测序分析显示,安装前的样本中可检测出大量皮氏罗尔斯顿菌、耐金属贪铜菌、奥斯陆莫拉菌、日本慢生根瘤菌、水生甲基杆菌、戈登分枝杆菌、新鞘氨醇杆菌等细菌序列,消毒后主要为宏基因测序分析常见的背景细菌。结论 持续性次氯酸灌注消毒可有效清除水管路中的细菌,并降低水管路中生物膜的形成,提升内镜终末漂洗水的质量。

Disinfection effect of persistent hypochlorous acid on final rinsing water for endoscope

Objective To discuss the disinfection effect of persistent hypochlorous acid supply to final rinse water for endoscope. Methods Water specimens of final rinse water for endoscope were collected at baseline, and identified by microbial culture and metagenomic next-generation sequencing (mNGS). After introducing a slightly acidic hypochlorous acid water generator, water specimens were collected at a specific time after installation for culture and mNGS analysis. Results A total of 27 water specimens were collected before installation, and 10 (37.03%) were qualified. Aquatic bacteria such as Rhizobiumradiobacter, Stenotrophomonas maltophilia, Microbacterium flavum, Cupriavidus, Methylobacterium fujisawaense and Sphingomonas paucimobilis can be detected in unqualified water specimens, 54 water specimens were collected after installation, and all of them were qualified. mNGS shows that abundant sequences of Ralstonia Pichia, Cupriavidus metallidurans, Moraxella osloensis, Rhizobium japonicum, Aquatic methylbacterium, Mycobacterium Gordon and Sphingosine neobacterium could be detected in water specimens before installation, but only background bacteria in mNGS were found after disinfection. Conclusion Persistent hypochlorous acid supply can effectively remove bacteria and reduce biofilm formation in water pipeline, improving the quality of final rinse water for endoscope.