痰涂片显微镜检查与培养结果的相关性分析 FREE

目的分析痰涂片显微镜检查（镜检）与培养结果的相关性。方法痰标本在进行细菌培养之前行涂片革兰染色、镜检；对培养出来的细菌进行革兰染色、镜检，对二者镜下染色的性质和形态进行比对；二者一致者作为病原菌进行下一步的鉴定和药敏试验。结果786份合格标本共检出病原菌540株，阳性率68.70%，其中革兰阳性球菌185株，革兰阴性杆菌316株，真菌39株。痰涂片镜检与培养鉴定结果的符合率为72.01%（566/786），其诊断敏感性为77.78%,特异性为59.35%,阳性预测值为80.77%，阴性预测值为54.89%。结论痰涂片镜检结果与培养结果具有较高的符合率，痰涂片镜检可以提高痰标本病原菌的检出率。

Correlation between sputum smear examination and bacterial culture results FREE

Objective To analyze the correlation between sputum smear examination （microscopy） and bacterial culture results. Methods Sputum specimens were performed sputum smear and Gram＇s staining before bacterial culture , and sputum cultured bacteria were also performed sputum smear and Gram＇s staining, then observed under microscope. The staining characteristics and appearance of two groups were evaluated; then further identification and antimicrobial susceptibility tests were carried out. Results Five hundred and forty pathogens were isolated from 786 qualified samples, the positive rate was 68. 70% there were 185 strains of Gram-positive cocci, 316 strains of Gram-negative bacilli and 39 strains of fungi. The coincidence between the results of two groups was 72. 01%（566/786）. The sensitivity of sputum smear was 77. 78%, specificity 59. 35%, positive predictive value 80. 77%, negative predictive value 54. 89%. Conclusion There is a high coincidence rate between the results of sputum smear and bacterial culture, which can improve the detection rate of pathogenic bacteria.