临海市蚊、蝇、蜚蠊对7种杀虫剂的抗性调查

目的了解临海市淡色库蚊、家蝇、德国小蠊现场品系（自然品系）对7种杀虫剂的抗性动态，为杀虫剂使用的选择和优化组合，指导灭蚊、蝇、蜚蠊工作的深入开展提供依据。方法药液浸渍法，测定4龄期蚊幼虫半数致死浓度；点滴法，测定家蝇半数致死量；三角烧瓶药膜法，测定德国小蠊半数击倒时间。结果淡色库蚊自然品系对仲丁威、三氯杀虫酯、溴氰菊酯、二氯苯醚菊酯的抗性倍数在1．89～8．93倍之间，属低抗水平（RR〈10），而敌敌畏的抗性倍数为10．16倍，属中抗水平（RR〉10）；家蝇自然品系对三氯杀虫酯和二氯苯醚菊酯的抗性倍数分别为1．26和5．32倍，属低抗水平（RR〈10），敌敌畏和高效氯氰菊酯的抗性倍数分别为17．44和12．64倍，属中抗水平（RR〉10），溴氰菊酯抗性倍数为37．86倍，属高抗水平（RR〉30）；德国小蠊自然品系对4种杀虫剂抗性测定结果，经统计学处理差异无统计学意义，抗性水平在2．53～4．12倍之间。结论淡色库蚊、家蝇和德国小蠊的自然品系对7种杀虫剂存在不同程度的抗药性。

Investigation on resistances to 7 insecticides of mosquito,fly and cockroach in Linhai

Objective To explore the resistance trends of Culex pipiens pallens, Musca domestica and Blattella germanica （field population） to 7 insecticides in Linhai and provide evidences for optimizing insecticides application and promoting insect control. Methods Dipping method was applied to the determination of LC50 for the fourth instar larva of C. pipiens pallens, topical application method for determination of LD50 for M. domestica and flask film method for determination of KT50 for B. germanica. Results The resistance of C. pipiens pallens field population to bassa, acetophenate, deltamethrin and permethrin were at low level with the range from 1.89 to 8.93 （RR〈 10）, and the resistance to dichlorovos was at medium level with the value of 10.16 （RR〉 10）. The resistance of M. dornestica field population to acetophenate and permethrin were at low level with the values of 1.26 and 5.32 （RR〈 10）, and the resistance to dichlorovos and cypermethrin were at medium level with the value of 17.44 and 12.64 （RR〉 10）. The resistance to deltamethrin were at high level with the value of 37.86 （RR〉 30）. The resistances of B. german ica field population to 4 inseticides were all at low level （RR = 2.53- 4.12） and had no significant differences among them. Conclusion The field population of mosquito, fly and cockroach had different resistances to all 7 insecticides.