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羧酸酯酶与北京市不同城区德国小蠊对有机磷杀虫剂抗性的关系
引用本文:魏绪强,钱坤,曾晓芃,高希武.羧酸酯酶与北京市不同城区德国小蠊对有机磷杀虫剂抗性的关系[J].中国媒介生物学及控制杂志,2008,19(5):413-417.
作者姓名:魏绪强  钱坤  曾晓芃  高希武
作者单位:1. 中国农业大学农学与生物技术学院昆虫系,北京,100094;北京市疾病预防控制中心消毒与有害生物防制所,北京,100013
2. 北京市疾病预防控制中心消毒与有害生物防制所,北京,100013
3. 中国农业大学农学与生物技术学院昆虫系,北京,100094
摘    要:目的研究羧酸酯酶在不同品系德国小蠊中的活性以及其在有机磷杀虫剂抗性形成中的作用。方法以对有机磷杀虫剂敏感品系(SS)及采自北京市宣武区(XW)、昌平区(CP)、顺义区(SY)和石景山区(SJS)的德国小蠊野外品系为试虫材料,利用生物测定、生化分析及增效剂试验等方法研究羧酸酯酶与德国小蠊对有机磷杀虫剂抗性的关系。结果增效剂TPP对马拉硫磷、敌敌畏的增效比在敏感品系中分别为1.86和1.51倍,TPP对马拉硫磷的增效比在XW、CP、SY和SJS品系中分别为5.79、20.08、15.26和4.74倍;TPP对敌敌畏的增效比在XW、CP、SY和SJS品系中分别为2.00、1.67、2.16和4.81倍。德国小蠊野外品系与敏感品系羧酸酯酶的米氏常数(Km)和最大反应速度(Vmax)之间差异有统计学意义,敏感品系羧酸酯酶的Km、Vmax分别为0.1580mmol/L和58.4225μmol/(mg pro·min);野外品系(XW、CP、SY和SJS)羧酸酯酶的Km、Vmax分别为0.1279、0.1071、0.1080、0.1095mmol/L和307.2550、338.5755、340.3300、212.4570μmol/(mg pro·min),2个品系的羧酸酯酶对马拉硫磷和敌敌畏的抑制作用也不同,2种有机磷药剂对敏感品系的抑制中量150分别为17.64mmol/L和0.91μmol/L,对野外品系(XW、CP、SY和SJS)的150分别为80.48、35.49、83.24、82.29mmol/L和15.35、7.89、11.52、8.60μmol/L。结论羧酸酯酶解毒代谢活性的增加是德国小蠊对有机磷杀虫剂产生抗性的机制之一。

关 键 词:德国小蠊  羧酸酯酶  有机磷杀虫剂  抗性机制

Relationship between carboxylesterase in German cockroach(Blattella germanica)from different areas in Beijing and its resistance to organophosphate
WEI Xu-qiang,QIAN Kun,ZENG Xiao-peng,GAO Xi-wu.Relationship between carboxylesterase in German cockroach(Blattella germanica)from different areas in Beijing and its resistance to organophosphate[J].Chinese Journal of Vector Biology and Control,2008,19(5):413-417.
Authors:WEI Xu-qiang  QIAN Kun  ZENG Xiao-peng  GAO Xi-wu
Institution:WEI Xu-qiang, QIAN Kun, ZENG Xiao-peng, GAO Xi-wu( 1 Department of Entomology, China Agricultural University, Beijing 100094, China; 2. Department of Disinfection and Vector Control, Beijing Center for Disease Control and Prevention, Beijing 100013, China)
Abstract:Objective To study the role of earboxylesterase in the development of organophosphate resistance and the activity variance of carboxylesterase in different strains of German cockroach (BlatteUa germanica ). Methods Relationship between carboxylesterase and organophosphate resistance in German cockroach was studied with bioassay, biochemical analysis and synergistic test. The cockroaches from the susceptible strain(SS) of laboratory and field strains of different areas in Beijing Xuanwu(XW) district, Changping(CP) district, Shunyi (SY) district and Shi)ingshan (SJS) district ] were used as test insects. Results The synergistic test showed that synergistic ratio of TPP to malathion and dichlorvos were 1.86 and 1.51 fold in susceptible strain, respectively. And in field strains (XW, CP, SY and SJS), they were 5.79, 20.08, 15.26 and 4.74 fold for malathion, respectively and 2.00, 1.67, 2.16 and 4.81 fold for dichlorvoa, respectively. There was significant difference between the Michaelis-Menten constants (Kin) and the maximum velocity ( Vmax ) Of carboxylesterase from two strains of German cockroach. The Km and Vmax of caboxylesterase in susceptible strain were 0.1580 mmol/L and 58.4225 μmol/(mg pro·min), respectively. And in field strains (XW, CP, SY and SJS), the Km and Vmax of carboxylesterase were 0.1279, 0.1071, 0.1080, 0.1095mmol/L and 307.2550, 338.5755, 340.3300, 212.4570 μmol/(mg pro·min), respectively. The inhibition medium concentration (I50) of malathion and dichlorvos to caroxylesterase from KS strain were 17.64 mmol/L and 0.91 μmol/L, respectively. For field strains, the I50 of malathion were 80.48, 35.49, 83.24 and 82.29 mmol/L, respectively, and that of dichlorvos were 15.35, 7.89, 11.52 and 8.60 μmol/L respectively. The inhibition of malathion and dichlorvos to caroxylesterase from KS strain (I50 were) was much lower than from field strains strain (XW, CP, SY and SJS). Conclusion The enhancement of detoxification activity was one of
Keywords:German cockroach(Blattella germanica)  Carboxylesterase  Organophosphate  Resistance mechanism
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