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| 云南省东北等地区蚊虫及蚊媒病毒调查研究 | |
| 引用本文： | 杨杜鹃,付士红,张海林,杨卫红,冯云,王静林,章域震,王丕玉,陈维欣,梁国栋.云南省东北等地区蚊虫及蚊媒病毒调查研究[J].中国媒介生物学及控制杂志,2011,22(4):304-308,312. |
| 作者姓名： | 杨杜鹃 付士红 张海林 杨卫红 冯云 王静林 章域震 王丕玉 陈维欣 梁国栋 |
| 作者单位： | 1. 大理学院公共卫生学院,云南大理,671000 2. 中国疾病预防控制中心病毒病预防控制所病毒性脑炎室,北京,102206 3. 云南省地方病防治所/云南省病毒立克次体研究中心,大理,671000 4. 云南省寄生虫病防治所 |
| 基金项目： | 中美传染病合作项目(U19-GH000004); 中日健康基金(2002-2007); 国家自然科学基金(30560142)~~ |
| 摘 要： | 目的了解滇东北等地区蚊虫及蚊传虫媒病毒分布特点，为虫媒病毒病防治提供科学依据。方法2009年在滇东北等地区的6个县采集蚊虫标本；蚊虫经分类鉴定后，用细胞培养法分离病毒，对病毒分离物进行分子生物学鉴定。结果共采集到4属（库蚊、按蚊、阿蚊、伊蚊）24种18562只蚊虫，其中三带喙库蚊、中华按蚊为主要蚊种，构成比分别为58．37％和28．45％；从蚊虫标本中分离到15株病毒，经分子生物学鉴定，其中2株（YN0911和YN0967）为基因I型流行性乙型脑炎（乙脑）病毒，均分离自三带喙库蚊；1株（YN0922）为版纳病毒，分离自中华按蚊；12株为淡色库蚊浓核病毒，其中9株分离自三带喙库蚊，3株分离自中华按蚊。结论三带喙库蚊和中华按蚊为调查地区的优势蚊种，并携带乙脯病毒、版纳病毒和淡仁．库蚊浓核病毒：滇东北地区首次分离到乙腩病毒。 |
| 关 键 词： | 蚊科 虫媒病毒 脑炎病毒 日本 版纳病毒 浓核病毒亚科 |
Investigation of mosquitoes and mosquito-borne viruses in northeast Yunnan province |
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| YANG Du-juan,FU Shi-hong,ZHANG Hai-lin,YANG Wei-hong,FENG Yun,WANG Jing-lin,ZHANG Yu-zhen,WANG Pi-yu,CHEN Wei-xin,LIANG Guo-dong.Investigation of mosquitoes and mosquito-borne viruses in northeast Yunnan province[J].Chinese Journal of Vector Biology and Control,2011,22(4):304-308,312. | |
| Authors: | YANG Du-juan FU Shi-hong ZHANG Hai-lin YANG Wei-hong FENG Yun WANG Jing-lin ZHANG Yu-zhen WANG Pi-yu CHEN Wei-xin LIANG Guo-dong |
| Institution: | YANG Du-juan1,FU Shi-hong2,ZHANG Hai-lin3,YANG Wei-hong3,FENG Yun3,WANG Jing-lin3,ZHANG Yu-zhen3,WANG Pi-yu4,CHEN Wei-xin2,LIANG Guo-dong21 School of Public Health,Dali University,Dali 671000,Yunnan Province,China,2 Department of Viral Encephalitis,Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,3 Yunnan Institute of Endemic Disease Control and Prevention/ Research Center of Arbovirus and Rickettsia,Yu... |
| Abstract: | Objective To investigate the distribution patterns of mosquitoes and mosquito-borne viruses in Yunnan province to provide evidence for prevention and control of mosquito-borne diseases. Methods In 2009, mosquito samples were collected from 6 counties of Yunnan province. Mosquitoes were first classified, followed by cell culturing for viral isolation for molecular identification. Positive isolates were identified using SDS-PAGE and RT-PCR. The genomes of isolates were also, sequenced for phylogenetic analysi... |
| Keywords: | Culicidae Arboviruses Encephalitis virus Japanese Banna virus Densovirinae |
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