酯酶同工酶法在净普力灭蚊作用机制研究中的应用

目的 通过对净普力筛选的致倦库蚊体内酯酶同工酶量的变化研究，以揭示酯酶同工酶在净普力灭蚊中所起的作用。方法 不连续聚丙烯酰胺凝胶电泳法。结果 净普力处理组与空白对照组酯酶同工酶电泳均可见3条带（分别称之为A、B和C带），其迁移率分别为0．42、0．45和0．51，显示两组蚊虫都表达了3种不同的酯酶同工酶。采用4种不同的上样量和2种不同的取样方法所得的电泳结果具有一致性。处理组与对照组相比，明显可见A带和C带颜色加深，显示这两种酶表达量增加；而B带颜色变浅，显示该酶表达量减少。结论 酯酶同工酶法可用于灭蚊机制研究。

Isozyme Analysis in the Research of the Zymplex Against Mosquito

Objective To determine the role of the esterase in the effect of the Zymplex on Culex(Culex) pipinens pallens Coquillet,1898.Methods Polyacrylamide gel electrophoresis(PAGE).Results Both the treated groups and the control demonstrated three bands(named A,B,C) and showed the samiliar results in different treated methods.Their relative migration rate were 0.42,0.45,0.51 respectively.It also showed that in the treated groups the amount of the esterase of B and A and C increased and in the control groups the amount of the esterase of B and C increased compared with each other.Conclusion PAGE method can be used in the study of the mechanism of the enzyme product against mosquito.