2010年安徽省蚊虫标本分离到基因Ⅰ型流行性乙型脑炎病毒

目的在安徽省不同地区开展流行性乙型脑炎病毒(JEV)病原学调查,了解当地JEV的基因型别及分子特征。方法 2010年8月在安徽省阜阳、淮南、安庆市的3个标本采集点使用诱蚊灯采集蚊虫标本,通过组织培养法分离病毒,并对病毒分离物进行血清学和分子生物学鉴定;利用生物信息学技术对新分离病毒的序列进行分析,完成同源性和系统发生分析。结果采集到3属3种共计7651只蚊虫标本,通过组织培养技术分离得到11株病毒分离物,鉴定结果显示均为基因Ⅰ型JEV。安徽省JEV新分离株与基因Ⅰ型JEV的同源性最高,核苷酸同源性为96.8%～99.5%,氨基酸同源性为97.8%～100%。结论在安徽省首次分离到基因Ⅰ型JEV,与我国上海市及浙江省JEV分离株进化关系较近。

Isolation and identification of genotypeⅠ Japanese encephalitis virus from mosquitoes in Anhui province,China in 2010

Objective To carry out the etiological investigation of Japanese encephalitis virus(JEV) in Anhui province,China,and to determine the genotype and molecular characteristics of the JEV.Methods Mosquito samples were collected using mosquito lamps in Fuyang,Huainan,and Anqing,Anhui province in August 2010 and then used for virus isolation by tissue culture.The obtained virus isolates were identified by serological and molecular biological methods.Homology and phylogenetic analysis were performed on the viral sequences by bioinformatics.Results A total of 7651 mosquitoes(3 species,3 genera) were collected,and 11 virus strains were isolated from the mosquitoes.The newly isolated JEV strains had the highest homology with genotype Ⅰ JEV(96.8%-99.5% nucleotide homology and 97.8%-100% amino acid homology) and thus were identified as genotype Ⅰ JEV.Conclusion Genotype Ⅰ JEV strains were first isolated in Anhui province,China,and they have a close phylogenetic association with JEV strains isolated from Shanghai and Zhejiang province,China.