陕西省不同地区家蝇抗药性调查

目的了解陕西省安康、渭南、宝鸡、铜川市家蝇抗药性情况,指导科学合理地使用杀虫剂。方法现场试虫采自城区蝇类孳生场所,敏感试虫为实验室不接触杀虫剂的家蝇,采用点滴法,以丙酮为对照,检查不同药物浓度对应的试虫死亡率,用POLO软件分析获得半数致死量(LD50)。结果实验室家蝇对敌敌畏、双硫磷、DDT、残杀威、高效氯氰菊酯、溴氰菊酯、氯氰菊酯和氯菊酯的LD50依次为0.004、0.113、0.010、0.214、0.00307、0.00063、0.003和0.013μg/只。安康、渭南、宝鸡、铜川市的家蝇对8种常用杀虫剂均产生了不同程度的抗药性,对敌敌畏、双硫磷、DDT、残杀威、高效氯氰菊酯、溴氰菊酯、氯氰菊酯和氯菊酯的LD50分别为0.051～0.301、4.429～14.480、1.259～1.573、13.579～33.556、0.024～0.303、0.017～0.046、0.047～0.269和0.016～0.053μg/只;8种杀虫剂的平均LD50为0.028～23.549μg/只,抗性倍数为2.54～140.20倍,以DDT最高,氯菊酯最低。结论应采取科学的方法使用杀虫剂,延缓家蝇抗性增长。

Investigation of resistance of Musca domestica to insecticides in the four areas of Shaanxi province

Objective To understand the resistance of Musca domestica to insecticides in the four areas of Shaanxi province for providing evidence for scientific application of insecticides. Methods Insects were collected in the fields in urban areas where flies bred and sensitive insects were reared in laboratory without exposure to insecticides. Topical dropping method was used to determine the death rate by different drug concentrations, with LD50 obtained by POLO software analysis. Results The LD50 of sensitive insects to DDVP, temephos, DDT, propoxur, beta-cypermethrin, deltamethrin, cypermethrin and permethrin was 0.004, 0.113, 0.010, 0.214, 0.003 07, 0.000 63, 0.003 and 0.013 μg/♀, respectively. Different degrees of resistance of M. domestica to the eight commonly used insecticides were observed in Ankang, Weinan, Baoji, Tongchuan city of Shaanxi province. The LD50 of M. domestica in four areas to DDVP, temephos, DDT, propoxur, beta-cypermethrin, deltamethrin, cypermethrin and permethrin was 0.051-0.301, 4.429-14.480, 1.259-1.573, 13.579-33.556, 0.024-0.303, 0.017-0.046, 0.047-0.269 and 0.016-0.053 μg/♀, respectively, with the average LD50 to the eight insecticides from 0.028 to 23.549 μg/♀. The resistance ratio (R/S) was from 2.54 to 140.20, DDT being the highest and permethrin the lowest. Conclusion Insecticides should be applied scientifically to effectively curb the increase of resistance of M. domestica to the insecticides.