湖北省部分地区2009年蚊传虫媒病毒调查

目的调查湖北省部分地区蚊传虫媒病毒种类和分布状况。方法 2009年夏季在湖北省黄冈市武穴市和咸宁市通城县采集蚊虫标本,用组织培养法分离病毒,用血清学和分子生物学方法对阳性病毒分离物进行鉴定,利用生物信息学软件对新分离病毒进行序列同源性和系统进化分析。结果采集到3属5种9424只蚊虫标本,阳性4株(HBTC0913、HBTC0917、HBTC0919和HBTC0921),经血清学和分子生物学鉴定均为版纳病毒;版纳病毒第12节段分子进化分析显示,4株新分离版纳病毒与中国北京、云南和内蒙古地区以及越南的分离株处于同一亚群,而印度尼西亚分离株处于另外一个进化群中;新分离株与其他分离株相比,第12节段编码区核苷酸同源性为87.2%～89.8%,氨基酸同源性为86.1%～90.9%。结论在湖北省分离到版纳病毒,与中国云南毒株YN6的进化关系较近。

Investigation of mosquito-borne arboviruses in some regions of Hubei province,China in 2009

Objective To investigate the species and distribution patterns of mosquito-borne arboviruses in some regions of Hubei province,China.Methods Mosquito samples were collected in Wuxue county of Huanggang city and Tongcheng county of Xianning city in the summer of 2009.Viruses were isolated from the samples by tissue culture,and the obtained virus isolates were identified by serological and molecular biological methods.Sequence homology and phylogenetic analysis was performed on the isolated viruses using bioinformatics software.Results A total of 9424 mosquitoes,belonging to 5 species and 3 genera,were collected.Four virus strains(HBTC0913,HBTC0917,HBTC0919,and HBTC0921) were isolated and identified as Banna virus(BAV).According to the phylogenetic analysis of the 12th segment of BAV,the four strains were in the same subgroup as the isolates from Beijing,Yunnan,and Inner Mongolia,China as well as Vietnam,and but in a different subgroup from the isolates from Indonesia.Compared with previous isolates,the BAV strains showed nucleotide and amino acid homologies of 87.2%-89.8% and 86.1%-90.9%,respectively,according to the homology analysis of the coding region of the 12th segment.Conclusion BAV strains were first isolated in Hubei province,China.They have a close phylogenetic association with YN6 strain isolated in Yunnan,China.