2010-2011无年形我体国的东感北染林情区况蜱调中查嗜吞噬细胞

目的调查我国东北林区蜱标本中嗜吞噬细胞无形体的感染情况。方法2010-2011年在东北林区采集蜱标本,用聚合酶链反应方法扩增gltA基因片段,分析东北林区蜱中无形体的感染情况,代表阳性片段联用长片段16S rRNA进行扩增测序确认;并将所得序列与GenBank中已注册的序列进行比对分析。结果共检测蜱2293只,其中2010年1161只,阳性率4.91%,2011年1132只,阳性率4.24%,2年的感染率差异无统计学意义(χ2=0.373,P=0.830;χ2=1.789,P=0.409)。全沟硬蜱阳性率4.86%(91/1872),长角血蜱为3.41%(14/411),森林革蜱为0(0/10),3个蜱种的感染率差异无统计学意义(P>0.05)。测得的gltA序列中,NE-gltA-1与Hc346株(GenBank:GU935788)最相似,仅差2 bp,相似性99%;NE-gltA-2与MDJ-Ip92株(HQ396224)序列一致,相似性100%。与2个序列相似性最远的是ZJ-HGA-72株(DQ458811),相差53 bp,相似性84%。测得的16S rRNA序列与我国从东北鼠源分离株China-C-T(tGQ412339)最接近,相似性为99%,与Florida株(AF309867)相似性最远,相似性97%。结论我国东北林区蜱中普遍存在嗜吞噬细胞无形体的感染,全沟硬蜱和长角血蜱可能为无形体的媒介宿主。

Investigation on the infection of ticks with Anaplasma phagocytophilum in the Northeast China forest region from 2010 to 2011

Objective To investigate the infection rate of ticks with Anaplasma phagocytophilumin in the Northeast China forest region.Methods In 2010 and 2011,bulk ticks were collected by flagging vegetation of grassland or woodland from the Northeast China forest region.16S rRNA and gltA gene fragment of A.phagocytophilum in ticks were amplified with PCR,followed by sequence analysis and comparison with the corresponding parts of the sequences deposited in GenBank.Results As a whole,a total of 2293 ticks were tested,including 1161 in 2010 and 1132 in 2011,with the positive rates of A.phagocytophilum being 4.91% and 4.24%,respectively.The positive rates of Ixodes persulcatus,Haemaphysalis longicornis and Dermacentor silvarum were 4.86%(91/1872),3.41%(14/411) and 0(0/10) with no significant difference among them.The sequence of NE-gltA-1 had the highest similarity to that of Hc346(GenBank:GU935788) and so was NE-gltA-2 and MDJ-Ip92(HQ396224),with their homology with ZJ-HGA-72(DQ458811) amounting to 84%.The similarities of 16S rRNA gene with that of China-C-Tt(GQ412339) and Florida(AF309867) were 99% and 97%,respectively.Conclusion It is concluded that there exists the infection of ticks with A.phagocytophilum in the Northeast China forest region,and I.persulcatus and H.longicornis may be involved in the maintenance and transmission of A.phagocytophilum.