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德国小蠊磷酸酯酶及谷胱甘肽S-转移酶生化特性的变化与抗药性的关系研究
引用本文:马红梅,陈海婴,柳小青,陶卉英.德国小蠊磷酸酯酶及谷胱甘肽S-转移酶生化特性的变化与抗药性的关系研究[J].中国媒介生物学及控制杂志,2008,19(5):422-425.
作者姓名:马红梅  陈海婴  柳小青  陶卉英
作者单位:南昌市疾病预防控制中心消毒与生物媒介防制所,南昌,330006
摘    要:目的了解德国小蠊的抗药性与磷酸酯酶和谷胱甘肽S-转移酶(GSTs)生化特性变化的关系,以初步揭示其抗性机制。方法参照Bessey等的方法测定磷酸酯酶活性与动力学参数Km和Vmax。参照Clark、Kao和Booth等的方法测定GSTs活性与动力学参数Km和Vmax。结果敏感品系与野生品系德国小蠊的酸性磷酸酯酶活力分别为0.98和26.95nmol/(只·30min),比活力分别为1.45和1.59μmol/(mg pro·30min),Km值分别是26.14和0.89mmol/L,Vmax值分别是3.33和0.85nmol/(mg pro·30min);敏感品系与野生品系德国小蠊的碱性磷酸酯酶活力为(0.03±0.00)和(0.28±0.06)nmol/(只·30min),比活力分别为(0.33±0.00)和(0.37±0.00)μmol/(mg pro·30min),Km值分别是70.38和61.24mmol/L,Vmax值分别是16.20和14.00nmol/(mg pro·30min);敏感品系与野生品系德国小蠊GETs活力分别是0.13和0.47nmol/min,比活力分别为8.94和17.37nmol/(mg pro·30min);Km值分别是2.08和5.81mmol/L,Vmax值分别是0.08和0.17nmot/min。结论磷酸酯酶与GSTs在野外品系的抗性形成过程中起一定作用。

关 键 词:德国小蠊  磷酸酯酶  谷胱甘肽S-转移酶  抗药性

The relationship between the activities of glulathione S-transferase and phosphatase and pesticide resistance in German cockroaches(Blattella germanica)
MA Hong-mei,CHEN Hai-ying,LIU Xiao-qing,TAO Hui-ying.The relationship between the activities of glulathione S-transferase and phosphatase and pesticide resistance in German cockroaches(Blattella germanica)[J].Chinese Journal of Vector Biology and Control,2008,19(5):422-425.
Authors:MA Hong-mei  CHEN Hai-ying  LIU Xiao-qing  TAO Hui-ying
Institution:( Nanchang Center for Disease Control and Prevention, Nanchang 330006, China )
Abstract:Objective To study the relationship between the activities of glutathione S-transferase (GSTs) and phosphatase from German cockroach(Blattella germanica ) and its resistance, so as to reveal its resistance mechanism. Methods The activity of phosphatase and the values of the Michaelis-Menten constant (Km ) and the maximum velocity ( Vmax ) were determined according to the method of Bessey et al. The activity of glutathione s-transferase and the values of its Km and Vmax were measured according to the methods of Clark, Kao and Booth. Results The activity of acidic phosphatase from sensitive strain and field strains were 0.98 and 26.95 nmol/( insect·30 min)respectively, and the specific activities of them were 1.45 and 1.59 μmol/(mg pro·30 min). The Km values were 26.14 and 0.89 mmol/L, and the values of the Vmax were 3.33 and 0.85 nmol/(mg pro·30 min) respectively. The activity of alkaline phosphatase (ALP) from two strains were (0.03 ± 0.00) and (0.28±0.06)nmol/ (insect·30min), and their specific activities were (0.33±0.00) and (0.37±0.00)μmol/ (mg pro· 30 min) respectively. The values of the Km were 70.38 and 61.24 mmol/L, and the values of the Vmax were 16.20 and 14.00 nmol/(mg pro·30 min). The activity of GSTs from two strains were 0.13 and 0.47 nmol/min respectively,and the specific activities were 8.94 and 17.37 nmol/(mg pro·min) respectively. The values of the Km were 2.08 and 5.81 mmol/L, and the values of the Vmax were 0.08 and 0.17 nmol/min. Conclusion The activity change of glutathione s-transferase and phosphatase from field strain German cockroaches was related to the resistance.
Keywords:German cockroaches(Blattella germanica)  Phosphatase  Glutathione S-transferase  Resistance
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