|
|||||
|
|
| 女生初潮年龄提前与黄体生成素受体基因Asp578Gly突变 | |
| 引用本文： | 朱丁,钱红丹,徐勇,张敏婕,沈惠芬,刘起展.女生初潮年龄提前与黄体生成素受体基因Asp578Gly突变[J].中国学校卫生,2011,32(9):1028-1029,1031. |
| 作者姓名： | 朱丁 钱红丹 徐勇 张敏婕 沈惠芬 刘起展 |
| 作者单位： | 1. 南京医科大学公共卫生学院,江苏210029;无锡市疾病预防控制中心 2. 苏州大学医学部公共卫生学院 3. 南京医科大学公共卫生学院,江苏,210029 |
| 基金项目： | 国家自然科学基金，无锡市卫生局指导性课题 |
| 摘 要： | 目的研究黄体生成素受体(LHR)基因Asp578Gly突变与女生初潮年龄提前的关系,为青少年青春期发育研究提供依据。方法分层整群抽取无锡市177名初潮年龄提前(初潮年龄<11岁)女生,外周血提取DNA,用5’-CAC TGC TGG CTT TTT CAC TGTATT-3’和5’-TGA AGG CAG CTG AGA TGG CAA AAA-3’作引物,经常规PCR扩增后,用限制性内切酶MspⅠ消化,以检测LHR基因是否存在A1733G的碱基突变。结果初潮年龄提前组LH水平高于正常组(P<0.05),而两组FSH差异无统计学意义(P>0.05)。177名初潮年龄提前女生的LHR基因均未检测到Asp578Gly突变。结论 Asp578Gly突变与女性初潮年龄提前无直接关系。 |
| 关 键 词： | 月经初潮 促黄体激素 基因 突变 学生 |
Asp578 Gly mutation of luteinizing hormone receptor in girls with early menarche |
|
| ZHU Ding,QIAN Hong-dan,XU Yong,et al..Asp578 Gly mutation of luteinizing hormone receptor in girls with early menarche[J].Chinese Journal of School Health,2011,32(9):1028-1029,1031. | |
| Authors: | ZHU Ding QIAN Hong-dan XU Yong |
| Institution: | ZHU Ding*,QIAN Hong-dan,XU Yong,et al.* School of Public Health,Nanjing Medical University,Nanjing(210029),China |
| Abstract: | Objective To identify the relationship between early menarche and Asp578 Gly mutation of luteinizing hormone receptor(LHR).Methods A total of 177 girls defined as early menarche(age of menarche less than 11 years old) were as subjects.The peripheral blood samples were taken from every girl.Genomic DNA was amplified by polymerase chain reaction,using primers of 5'-CAC TGC TGG CTT TTT CAC TGT ATT-3' and 5'-TGA AGG CAG CTG AGA TGG CAA AAA-3'.Then,the PCR product was treated with Msp I to examine whether a tran... |
| Keywords: | Menarche Luteinizing hormone Gene Mutation Students |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |