低剂量鱼藤酮诱导BV2细胞IL-6产物及montelukast的作用

目的：在体外帕金森病（Parkinson's disease，PD）模型观察低剂量鱼藤酮诱导的BV2小胶质细胞白介素6（IL-6）生成及montelukast的作用。方法以PD诱导因子鱼藤酮处理小鼠小胶质细胞系BV2细胞。 Western blot方法检测BV2细胞前炎症细胞因子 IL-6蛋白表达， ELISA 方法检测 BV2细胞 IL-6释放。观察 CysLT1R 拮抗剂montelukast对鱼藤酮诱导的小胶质细胞IL-6的影响。结果低剂量鱼藤酮（0．3～3 nM）浓度依赖性地增加IL-6蛋白表达；以鱼藤酮（3 nM）处理细胞1～24h，时间依赖性的诱导IL-6释放增加。 CysLT1R拮抗剂montelukast降低鱼藤酮增高的BV2细胞IL-6水平。结论低剂量鱼藤酮诱导小胶质细胞前炎症细胞因子IL-6增加，montelukast抑制鱼藤酮诱导BV2细胞效应。

Low-dose rotenone-induced interleukin-6 production and the effect of montelukast in BV2 cells

Objective To understand low-dose rotenone-induced interleukin-6 ( IL-6 ) production and the effect of montelukast in an in vitro model of Parkinson's disease (PD).Method BV-2 cells, an immortalized cell line of murine microglial cells , were treated with PD inducer rotenone .The expression of proinflammatory cytokine ( IL-6 ) protein was detected with Western blot , and the level of IL-6 in the supernatants was analyzed by ELISA .The effect of CysLT1R antagonist montelukast on rotenone-induced microglial IL-6 production was investigated .Findings Treatment with low-dose rotenone (0.3-3 nM) dose-dependently increased cytokine IL-6 expression in BV2 cells.Exposure to (3 nM) rotenone for 1-24h induced IL-6 release from BV2 cells in a time-dependent manner .CysLT1R antagonist montelukast (0.1 and 1μM) significantly reduced IL-6 production in rotenone (3 nM)-treated BV2 cells.Conclusion Low-dose rotenone can induce microglial proinflammatory cytokine IL-6 production .Montelukast can effectively inhibit low-dose rotenone-induced microglial response .