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活性氧自由基在ARDS发病机制中作用的实验研究
引用本文:刘和亮,赵金垣.活性氧自由基在ARDS发病机制中作用的实验研究[J].中国工业医学杂志,2003,16(2):72-75.
作者姓名:刘和亮  赵金垣
作者单位:北京大学第三医院职业病研究中心,北京,100083
基金项目:国家自然科学基金课题 (编号 :30 1 70 799),国家教育部博士点基金课题 (编号 :2 0 0 1 0 0 0 1 0 92 )
摘    要:目的 探讨活性氧(ROS)自由基在急性呼吸窘迫综合征(ARDS)发病机制中的作用。方法 采用电子自旋共振(ESR)技术直接测量ROS,并结合超微弱发光法、化学发光法等技术分析鉴定了RPS的种类。结果 ESR结果显示,大鼠注射OA后10min ROS含量即升高,30min达到高峰,以后逐渐下降;利用超微弱发光法,投用不同ROS的清除剂得出OA型ARDS发病中起主要作用的ROS是超氧阴离子(02^-),而化学发光法定量检测结果与ESR波谱显示的结果完全一致。结论 ROS的改变早于肺毛细血管内皮(PCEC)损伤和病理形态的发生,ROS可能是ARDS启动的主要病因;OA型ARDS发病中起主要作用的ROS是02^-,今后在临床上可针对性应用SOD或SOD类似物来进行治疗。

关 键 词:活性氧  自由基  ARDS  发病机制  实验  ROS  急性呼吸窘迫综合征  电子自旋共振  化学发光法
文章编号:1002-221X(2003)02-0072-04
修稿时间:2002年12月25

Experimental study of reactive oxygen species in the pathogenesis of ARDS
LIU He-liang,ZHAO Jin-yuan.Experimental study of reactive oxygen species in the pathogenesis of ARDS[J].Chinese Journal of Industrial Medicine,2003,16(2):72-75.
Authors:LIU He-liang  ZHAO Jin-yuan
Abstract:Objective To investigate the role of reactive oxygen species(ROS)in the pathogenesis of acute respiratory distress syndrome(ARDS).Method ROS was measured by electron spin resonance(ESR)directly and the species of ROS were identified and quantified by the ultra-weak luminescence and chemical luminescence.Result The spectrum of ESR showed that the concentration of ROS increased at10minutes and got summit at30minutes after injection of oleic acid(OA),then dropped gradually;the scaveng-ing effect of different scavenging agent to ROS in the experiment of ultra-weak luminescence proved that superoxide anion play an impor-tant role in the development of ARDS induced by chemicals,moreover,the results of quantified measure to superoxide anion by chemi-cal luminescence corresponded with the tendency exhibited in ESR measurement.Conclusion The results demonstrated that the change of ROS was earlier than that of pulmonary capillary endothelial cells(PCEC)and pathophysiological change,ROS may be a key factor in the starting phase of ARDS and superoxide anion may be a leading actor in the action of ROS.It would be helpful to use superoxide dismutase(SOD)and the analog of SOD in the future clinical treatment of ARDS because SOD is the special scavenging agent to super-oxide anion.
Keywords:Reactive oxygen species  Acute respiratory distress syndrome  Electron spin resonance  Ultra-weak luminescence
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