| 烧伤病房铜绿假单胞菌的耐药检测及脉冲场凝胶电泳分析 |
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| 引用本文： | 胡锡浩,许小敏,金春光,陈丽娜.烧伤病房铜绿假单胞菌的耐药检测及脉冲场凝胶电泳分析[J].中华医院感染学杂志,2006,16(4):450-452. |
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| 作者姓名： | 胡锡浩 许小敏 金春光 陈丽娜 |
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| 作者单位： | 宁波市第二医院,浙江,宁波,315010 |
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| 摘 要： | 目的了解烧伤病房铜绿假单胞菌的耐药性,用脉冲场凝胶电泳对铜绿假单胞菌进行分子流行病学的调查。方法采用琼脂纸片扩散法对9种抗菌药物作敏感试验,其中20株进行脉冲场凝胶分析。结果20株PAE对9种抗菌药物显示很高的耐药率,多重耐药率为30%;20株菌经脉冲场凝胶分析分别有3组菌株具有同源性。结论铜绿假单胞菌对常用抗菌药物耐药率高,多重耐药情况严重,脉冲场凝胶电泳是研究铜绿假单胞菌分子流行病学较好的基因分型方法。
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| 关 键 词： | 铜绿假单胞菌 多重耐药性 烧伤病房 |
| 文章编号： | 1005-4529（2006）04-0450-03 |
| 收稿时间： | 2005-08-19 |
| 修稿时间： | 2005-11-18 |
Antibiotic Resistance and Genotyping of Pseudomonas aeruginosa Isolated from Burn Unit by Pulsed-field Gel Electrophoresis |
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| HU Xi-hao,XU Xiao-min,JIN Chun-guang,CHEN Li-na.Antibiotic Resistance and Genotyping of Pseudomonas aeruginosa Isolated from Burn Unit by Pulsed-field Gel Electrophoresis[J].Chinese Journal of Nosocomiology,2006,16(4):450-452. |
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| Authors: | HU Xi-hao XU Xiao-min JIN Chun-guang CHEN Li-na |
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| Institution: | Ningbo Second Hospital, Ningbo, Zhejiang 315010, China |
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| Abstract: | OBJECTIVE To analyze antibiotic resistance pattern and use pulsed-field gel electrophoresis(PFGE) to study the molecular epidemiology of Pseudomonas aeruginosa isolated from burn unit. METHODS P.aeruginosa had been isolated and tested by K-B method from clinical samples and antibiotic resistance was analyzed and studied retrospectively. RESULTS The drug resistance of P.aeruginosa to nine antibiotics was high,the multiple drug resistance rate was 30%. CONCLUSIONS The resistance rates to commonly used antibacterials in P.aeruginosa are high and the resistance pattern is wide.PEGE is a better genotyping method to study molecular epidemiology and analytic homology. |
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| Keywords: | PFGE |
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