乡村医疗机构与城市医院来源的艰难梭菌MLST分型对比研究

目的 初步了解农村社区艰难梭菌流行状况及分子生物学特征.方法 对河南省济源市某乡村医疗机构送检的7株艰难梭菌进行生化鉴定和分子生物学方法鉴定,并进行MLST分型分析,同时与北京某三甲医院的84株艰难梭菌进行对比,用eBURST软件聚类分析,寻找其中可能的关联.结果 源自河南济源市某乡村医院的7株菌均属艰难棱菌,PCR毒素基因鉴定结果显示,其中两株为A-B+菌株,其MLST分型结果是ST37;5株为A+B+菌株,其中4株对应ST35,1株对应ST3；两重来源的菌株中均是毒素基因A+B+菌株占优势；乡村医疗机构的7株艰难棱菌中未发现新的与David MLST数据库中不同的ST型别,未发现不同于北京城市医院的ST型别.结论 David方案适合种群结构和全球流行病学研究,且其数据库全球共享,便于数据交换；该次研究中未发现新的ST型别,所有菌株ST呈散在分布.

MLST typing of Clostridium difficileisolated from rural and urban China : a comparative study

OBJECTIVE To understand the epidemic status and molecular biological characteristics of Clostridium difficile in rural area of China.METHODS The biochemistry characteristics and molecular biological characteristics of the 7 strains of C.difficile isolated from rural clinics in China were identified,followed by the analysis of MLST,and then we compared the results with other 84 strains derived from a third-grade class-A hospital,Beijing.Finally,a preliminary discussion about the possible relationship between the two teams was made after cluster analysis via eBURST.RESULTS Of the 7 strains,2 strains which belonged to ST37 were A-B+,5 strains were A+B+,4 of which belonged to ST35 and 1 of which belonged to ST3.The A+B+ strains were predominant in both studies and no new STs were detected.CONCLUSION We adopt David’s MLST scheme because of the advantages for studing the group construction and global epidemiology and the globally accessible data;there are no new STs detected in the study,and all the ST strains scattered.