| 激活蛋白-1与p53基因启动子结合的染色质免疫沉淀技术分析 |
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| 引用本文： | SHEN Fei,李智涛,WU Yi-ming,吴卫东.激活蛋白-1与p53基因启动子结合的染色质免疫沉淀技术分析[J].中华劳动卫生职业病杂志,2008,26(8). |
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| 作者姓名： | SHEN Fei 李智涛 WU Yi-ming 吴卫东 |
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| 作者单位： | 1. Department of Occupational Health and Environmental Health,Public Health College,Zhengzhou University,Zhengzhou,Henan Province 450001,China
2. 郑州大学公共卫生学院职业卫生与职业医学教研室,河南,450001 |
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| 摘 要： | 目的 检测肺腺癌A549细胞内的转录因子激活蛋白-1(AP-1)与p53基因调控区的结合情况.方法 采用染色质免疫沉淀技术,用c-jun特异性抗体沉淀DNA,聚合酶链反应(PCR)技术检测p53基因5'端特异性序列.结果 在c-jun特异性抗体免疫沉淀的DNA片段中扩增出p53 5'端的特异性序列.结论 AP-1在A549细胞内结合于p53基因的调控区,参与该基因的表达调控.
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| 关 键 词： | 转录因子AP-1 基因 p53 沉淀素试验 |
Preliminary study of p53 gene promoter binding site of activator protein-1 in A549 cell lines by chromatin immunoprecitation |
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| SHEN Fei,LI Zhi-tao,WU Yi-ming,WU Wei-dong.Preliminary study of p53 gene promoter binding site of activator protein-1 in A549 cell lines by chromatin immunoprecitation[J].Chinese Journal of Industrial Hygiene and Occupational Diseases,2008,26(8). |
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| Authors: | SHEN Fei LI Zhi-tao WU Yi-ming WU Wei-dong |
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| Abstract: | Objective To detect and verify activator protein-1(AP-1)binding to p53 gene promoter region in A549 cell lines in vitro.Methods AP-1 binding to p53 gene promoter region was detected by chromatin immunoprecipitaLion (ChIP)assay using c-jun specific antibody,and PCR amplified its gene specific DNA fragment.Results The p53 gene specific fragment was found in the DNA fragment immunoprecipitated by c-jun antibody.Conclusion AP-1 binds to p53 gene promoter region in A549 cells,and regulates its expression. |
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