镍冶炼烟尘诱导哺乳动物细胞遗传物质损伤作用的研究

目的 研究2种镍冶炼烟尘致细胞毒性和DNA损伤的作用及其差异.方法 以某镍冶炼厂的镍冶炼炉前沉降尘和镍精炼车间沉降尘为受试物,用噻唑蓝(MTT)染色的方法 检测(受试物浓度分别为100.00、50.00、25.00、12.50、6.25、0.00μg/ml)作用6、12、18、24、36、48 h的细胞相对存活率;单细胞凝胶电泳技术检测(受试物浓度分别为100.0、50.0、25.0、12.5、0.0μg/ml))作用2、4、8 h其致细胞的DNA损伤.结果 随着染毒时间的延长和染毒剂量的增加,细胞存活率下降;2种冶炼烟尘引起的细胞拖尾率、尾矩和细胞尾部DNA含量增加.在浓度为100.00μg/ml的2种烟尘作用细胞48 h后,细胞的存活率仅为24.5%和26.5%;镍冶炼炉前沉降尘的各浓度组在不同时间诱导的细胞拖尾率和尾部DNA含量均高于阴性对照组,差异有统计学意义(P<0.05),镍精炼车间沉降尘除12.5μg/ml各作用时间组和50.0μg/ml作用2 h组外的各实验组在不同时间诱导的细胞拖尾率和尾部DNA含量均高于阴性对照组,差异有统计学意义(P<0.05).结论 镍冶炼烟尘可以降低细胞的相对存活率,并不同程度地致NIH/3T3细胞的DNA损伤.

Genetic damage of mammalian cells induced by nickel-refining fumes

Objective To study the effects of two kinds of nickel-refining fumes on DNA damage of NIH/3T3 cell and the difference. Methods NIH/3T3 cells were treated by two kinds of nickel fumes collected from smelting furnace and refining workshop of a nickel-smeltery, and PBS taken the place of nickel-smelting fumes was used as negative control. Several hours later, the cytotoxicity of on NIH/3T3 cells was detected with MTT colorimetrie assay, and the DNA damage was also measured by comet assay (single cell gel elec-trophoresis). Result With the extension of exposure time and increasing of concentration, the living rate of NIH/3T3 cells was decreased; the tail rate, tail extent moment and tail DNA percent of NIH/3T3 cell induced by these two refining fumes were increased. After cells were treated with 100.00 μg/ml of nickel-smelting fume for 48 h, the living rate of NIH/3T3 cells was 24.5% and 26.5% respectively. The tail length of NIH/3T3 cell induced by these two refining fumes was not significant difference. Tail DNA percent of NIH/3T3 cell induced by smelting furnace fume was higher than negative control group(P<0.05). The tail rate, and tail DNA percent (except 12.5 μg/ml and 50.0 μg/ml treated 2 h group) of NIH/3T3 cell induced by refining wokshop fume was higher than negative control group (P<0.05). Conclusion Nickel-smelting fume could depress the survival rate of NIH/3T3 ceils ,and induce different degree DNA damage of NIH/3T3 cell.