红芸豆植物凝集素凝胶剂杀精与凝集精子效果的实验研究

目的:探讨红芸豆植物凝集素(RKBL)凝胶剂的杀精和凝集精子的效果。方法:(1)采用Sander-Cramer方法,即在浓度为5,2.5,1,0.5,0.25,0.1,0.05,0.025,0.01,0.005,0.0025,0.001mg/ml的RKBL溶液中,分别加入等体积精液,观察20s内致死精子的最低RKBL浓度及精子凝集的有效浓度;(2)在"V"型管底部加入等体积RKBL浓度分别为5,2.5,1,0.5,0.25,0.1,0.05,0.025,0.01,0.005,0.0025,0.001mg/ml的凝胶剂,在V型管两侧加入等体积人输卵管培养液(HTF),然后在其中一侧HTF中加入等量精液,观察阻止精子穿过或致死精子的效果。结果:当RKBL的浓度≥1mg/ml时,精子瞬间失活致死,即20s内致死的最低剂量为1mg/ml;0.01mg/ml的各浓度RKBL可不同程度地凝集精子。在"V"形管中,上述不同浓度的RKBL凝胶剂均能阻止精子穿透;当RKBL浓度≥1mg/ml时,精子在凝胶附近完全失去活动能力。结论:RKBL溶液体外杀精的最低浓度为1mg/ml;部分低浓度RBKL能使精子产生不同程度的凝集;各实验浓度的RKBL凝胶剂均能阻止精子穿透。RKBL凝胶剂可有效用于避孕。

Experimental study of spermicidal and sperm agglutinated efficacy of red kidney bean lectin gel

Objective:To explore the spermicidal and sperm agglutinated efficacy of red kidney bean lectin (RKBL).Methods:① Sander Cramer test has been used to observe the spermicidal efficacy when mixed 5,1,0.5,0.25,2.5,0.1,0.05,0.025,0.01,0.005,0.0025,0.001 mg/ml of RKBL gels with the same volume of semen in 20s,and the lowest dose of RKBL which killed all sperm in 20s was also explored,and the effective concentration of RKBL on inducing sperm agglutination was also observed.②5,1,0.5,0.25,2.5,0.1,0.05,0.025,0.01,0.005,0.0025,0.001 mg/ml of RKBL gels were put into the bottom of the "V" pipe,then added equal volume HTF medium to both side of the "V" type pipe.After that,Sperm was added into one side of the pipe,and then the effect of stopping sperm penetration and killed sperm were observed.Results:when the RKBL gel concentration was 1mg/ml or above,sperm was inactivation and instant death,so the lowest dose for effective killing sperm by the Sander Cramer test in 20s was 1mg/ml.Sperm agglutination had occurred when RKBL gel concentration was 0.01mg/ml or above.In "V" type pipe,different concentrations of RKBL gel could prevent sperm penetration.When the concentration of RKBL gel was 1mg/ml or above,the sperm completely lost activity near the gel.Conclusion:The lowest effective concentration of RKBL gel for killing sperm is 1mg/ml or above.Some low concentration of RBKL gel can induce sperm aggregate in different degrees.The different concentration of RBKL gel can prevent sperm penetration effectively.RKBL gel can be used as a contraception.