| 保健食品耐缺氧功能体外评价方法建立 |
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| 引用本文： | 王凤岩,黄俊明,谭剑斌,胡永成,杨颖,李欣,陈瑞仪.保健食品耐缺氧功能体外评价方法建立[J].中国公共卫生,2011,27(5). |
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| 作者姓名： | 王凤岩 黄俊明 谭剑斌 胡永成 杨颖 李欣 陈瑞仪 |
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| 作者单位： | 广东省疾病预防控制中心卫生毒理所,广州,510300 |
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| 基金项目： | 广东省医学科研基金(B2005004) |
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| 摘 要： | 目的将小鼠大脑皮质神经元的缺糖/缺氧模型用于耐缺氧保健食品的体外评价。方法采用血清药理学方法制备含牛磺酸复合制剂的小鼠血清,观察其对缺糖缺氧损伤神经元的乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)活力及细胞生存率的影响,并与小鼠急性脑缺血性缺氧实验结果比较。结果中、高剂量含牛磺酸复合制剂小鼠血清组LDH活力分别为(5.97±0.85)、(5.68±1.55)U/(g.prot),低于空白血清对照组的(8.27±1.54)U/(g.prot)(P<0.05);低、中、高剂量含牛磺酸复合制剂小鼠血清组SOD活力分别为(6.83±0.65)、(6.95±0.68)、(7.55±0.82)U/(mg.prot),高于空白血清对照组的(5.57±0.89)U/(mg.prot),细胞生存率分别为90.06%、89.04%、91.92%,高于空白血清对照组的75.74%(P<0.05);牛磺酸复合制剂低、中、高剂量组小鼠急性脑缺血性缺氧存活时间分别为17.3、19.5、18.2 s,比纯水对照组的15.5 s长(P<0.05)。结论含有某保健食品的小鼠血清对神经元缺糖/缺氧损伤具有保护作用;小鼠大脑皮质神经元的缺糖/缺...
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| 关 键 词： | 神经元 缺糖/缺氧模型 血清药理学 |
Development of neuron glucose oxygen deprivation model in evaluation on anoxia endurance function of health food |
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| WANG Feng-yan,HUANG Jun-ming,TAN Jian-bin,et al..Development of neuron glucose oxygen deprivation model in evaluation on anoxia endurance function of health food[J].Chinese Journal of Public Health,2011,27(5). |
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| Authors: | WANG Feng-yan HUANG Jun-ming TAN Jian-bin |
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| Institution: | WANG Feng-yan,HUANG Jun-ming,TAN Jian-bin,et al.Department of Toxicology,Center for Disease Prevention and Control of Guangdong Province(Guangzhou 510300,China) |
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| Abstract: | Objective To develop a mouse cortical neuron glucose oxygen deprivation model to evaluate anoxia endurance(acute hypoxic-ischemic damage)function of health food in vitro.Methods Serum pharmacology method was adopted to prepare mouse serum containing taurine.Primary cortical neurons from neonate mouse(within 24hr)were cultured and treated with different concentrations of the serum on the 8th day under the deprivation of oxygen and glucose.Then the contents of lactate dehydrogenase(LDH) and superoxide dismuta... |
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| Keywords: | neuron oxygen/glucose deprivation model serum pharmacology |
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