| 蝇蛆壳聚糖对H2O2致内皮细胞损伤保护作用 |
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| 引用本文： | 覃容贵,吴建伟,国果,付萍. 蝇蛆壳聚糖对H2O2致内皮细胞损伤保护作用[J]. 中国公共卫生, 2011, 27(10) |
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| 作者姓名： | 覃容贵 吴建伟 国果 付萍 |
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| 作者单位： | 1. 贵阳医学院药学院药用植物学与生药学教研室,贵州贵阳,550004
2. 贵阳医学院基础医学院 |
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| 基金项目： | 贵州省重点技术创新项目 |
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| 摘 要： | 目的 研究蝇蛆壳聚糖(HMC)对过氧化氧(H2O2)诱导的内皮细胞氧化应激损伤的保护作用.方法利用H2 O2诱导的内皮细胞损伤模型,采用不同剂量HMC预培养,通过四甲基偶氮噻唑蓝(MTT)比色法检测细胞存活率,生化法和放免法检测细胞培养液超氧化物歧化酶(SOD)和丙二醛(MDA)、一氧化氮(NO)和内皮素(ET)水平.结果中、高剂量HMC组细胞存活率A值分别为(0.714 0±0.059 5)、(0.811 9±0.059 7),均高于H2O2损伤组(0.5972±0.042 2) (P ＜0.01)；低、中、高剂量HMC组细胞SOD活性分别为(14.76±1.59)、(16.99±1.69)、( 19.22±2.31) NU/mL,明显高于H2O2损伤组(P＜0.05,P＜0.01)；低、中、高剂量HMC组细胞MDA含量分别为(10.11 +1.91)、(8.23±1.64)、(6.74±1.26) nmol/L,明显低于H2 O2损伤组(P＜0.01)；各剂量HMC组细胞NO水平明显高于H2 O2损伤组,而ET含量则明显低于H2 O2损伤组(P＜0.01).结论HMC具有保护血管内皮细胞的作用,机制可能与减少细胞NO释放、增加ET水平、维护内皮细胞结构和功能完整及增强其抗氧化能力有关.
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| 关 键 词： | 蝇蛆壳聚糖 小鼠脑微血管内皮细胞 氧化应激 |
Protective effect of housefly maggot chitosan on brain microvascular endotheliocyte |
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| QIN Rong-gui,WU Jian-wei,GUO Guo,et al.. Protective effect of housefly maggot chitosan on brain microvascular endotheliocyte[J]. Chinese Journal of Public Health, 2011, 27(10) |
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| Authors: | QIN Rong-gui WU Jian-wei GUO Guo et al. |
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| Affiliation: | QIN Rong-gui,WU Jian-wei,GUO Guo,et al.Depaerment of Medicinal Botany and Pharmacognosy,Guiyang Medical University(Guizhou 550004,China) |
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| Abstract: | Objective To study the protective effect of housefly maggot chitosan(HMC) on the damage of brain microvascular endotheliocyte induced by H2O2 in vitro.Methods Normal cells,damaged cells induced by H2O2 were cultured in modified minimal essential medium(DMEM),together with different doses of HMC.The survival rates of the cells were tested with 3-(4,5 dimethythiazol-2-yl)-2,5 diphenyl-tetrazolium bromide(MTT)assay.Vasoactive substance contents such as nitric oxide(NO) and endothelin(ET)in cell culture fluid w... |
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| Keywords: | housefly maggot chitosan mouse brain microvascular endotheliocyte oxidation stress |
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