甲基苯丙胺对大鼠小胶质细胞损伤作用

目的观察甲基苯丙胺(Meth)通过电压门控钾离子通道亚型1.3、1.5(Kv1.3、Kv1.5)对大鼠胎鼠小胶质细胞的损伤作用。方法SD胎鼠原代培养小胶质细胞,细胞计数试剂盒(CCK-8)检测细胞活力和原位末端转移酶标记技术(TUNEL)检测细胞凋亡;采用蛋白质印迹法和实时荧光定量聚合酶链反应法观察Kv1.3、Kv1.5表达变化。结果Meth呈浓度依赖性降低小胶质细胞活力,增加小胶质细胞凋亡;Kv1.3的抑制剂MgTx对Meth所致小胶质细胞损伤有部分保护作用;与对照组(1.047±0.165)比较,300 μmol/L Meth组小胶质细胞Kv1.3 mRNA表达(7.453±0.675)增高(P<0.05),MgTx组Kv1.3 mRNA表达(1.684±0.875)低于Meth 300 μmol/L组(P<0.05);Meth对Kv1.5 mRNA表达无影响。结论Meth可引起小胶质细胞损伤,其机制可能与Kv1.3表达变化有关。

Effect of methamphetamine on microglia cells of rats through potassium channel subtype Kv1.3 and Kv1.5

ObjectiveTo investigate the effect of methamphetamine (Meth) on the expression of voltage-gated potassium channel subtype 1.3 and 1.5 (Kv1.3 and Kv1.5) with primary cultured fetal rat cortical microglia. MethodsThe cell viability and apoptosis were detected with CCK-8 as well as terminal deoxyribonucleotidyl transferase (TdT)-mediated biotin-16-dUTP nick-end labeling (TUNEL) assay.The expression of Kv1.3 and Kv1.5 mediated by Meth was evaluated with real-time PCR and western blot. ResultsMeth reduced cell viability and increased cell apoptosis in a dose-dependent manner.Compared with that of the control(1.047?0.165),Meth could increase the mRNA expression of Kv1.3(7.453?0.675).But this process could be partly retarded by MgTx(1.684?0.875).The mRNA expression of Kv1.3,but not Kv1.5,was up-regulated by Meth. ConclusionKv1.3 is involved in Meth mediated microglia cell injury.