| 依布哂啉纯度、杂质检测及安全性实验 |
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| 引用本文： | 张静,谢虹,赵美英,林凯,张红宇.依布哂啉纯度、杂质检测及安全性实验[J].中国公共卫生,2004,20(7):823-824. |
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| 作者姓名： | 张静 谢虹 赵美英 林凯 张红宇 |
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| 作者单位： | 1. 深圳市疾病预防控制中心,518020
2. 华中科技大学同济医院院劳动及环境卫生学教研室 |
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| 基金项目： | 深圳市卫生科技项目 (2 0 0 30 4 1 64) |
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| 摘 要： | 目的 检测合成的依布哂啉(Ebselen Ebs)的纯度、杂质并进行急性毒性实验。方法 Ebs测定采用自建立的高效液相色谱法(HPLC)，杂质(苯胺)采用比色法，急性毒性试验采用成年大鼠1次性灌胃，观察24h内死亡率。结果 Ebs纯度为99.95％～99．99％，苯胺含量为0．00017％～0．00019％，大鼠1次经口的LD50为2355．05mg／kg。结论 Ebs质量可靠，安全性高，可供开发研究。
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| 关 键 词： | Ebselen 纯度 HPLC 杂质 苯胺 LD50 |
| 文章编号： | 1001-0580(2004)07-0823-02 |
| 修稿时间： | 2004年2月5日 |
Detection of purity and impurity of ebselen and security test |
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| ZHANG Jing,XIE Hong,ZHAO Mei-ying,et al..Detection of purity and impurity of ebselen and security test[J].Chinese Journal of Public Health,2004,20(7):823-824. |
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| Authors: | ZHANG Jing XIE Hong ZHAO Mei-ying |
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| Institution: | ZHANG Jing,XIE Hong,ZHAO Mei-ying,et al.Shenzhen Center for Disease Control and Prevention |
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| Abstract: | Objective To understand the purity and the impurity of the synthesized ebselen(Ebs) and the acute toxicity.Methods The Ebs was tested with HPLC,the impurity was detected with chromatometry and the acute test was done by adult rat lavaged one time and the 24-hour mortality rate was observed.Results The purity of Ebs was 99.95%-99.99%,the amount of phenylamine was 0.000 17%-0.000 19%,the LD 50 of the rat lavaged by mouth one time was 2 355.05 mg/kg.Conclusion The quality of Ebs was reliable,the security was high,and it could be used for exploitation and study. |
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| Keywords: | ebselen purity HPLC impurity phenylamine LD50 |
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