HLA-DR抗原与HBV宫内感染关系研究

目的 了解人类白细胞抗原-DR(HLA-DR)抗原与HBV宫内感染的关系，探索HBV宫内感染的遗传易感性。方法以1997年2月～1998年8月在太原市传染病医院分娩的HBsAg阳性孕妇新生儿为研究对象．随机选择发生及未发生HBV宫内感染的新生儿各20例，采用序列特异引物PCR(PCR-sequencelspecificprimer，PCR-SSP)技术扩增HLA-DR基因区18对等位基因。判定HLA-DR抗原的型别。计算HLA-DR抗原各型别出现频率并分析其在2组间的差别。结果 DR3、DR51在宫内感染组的频率较高，分别为45％，40％，在非宫内感染组分别为20％。15％，OR值分别为3．27(0．67～17．08)、3．78(0．69～23．02)．P分别为0．18，0．16。DR53在非宫内感染组的频率较高．为60％，在宫内感染组为35％，OR值为0．36(0、08～1．54)P为0．20，均未达显性差异。结论 DR3、DR51有可能是HBV宫内感染的易感型．DR53有可能具有保护作用．但需要扩大样本量进一步研究。

Study on association between HLA-DR phenotype and intrauterine transmission of hepatitis B virus

Objective To study the association between HLA-DR phenotype and intrauterine transmission of hepatitis B virus to probe into the genetic susceptibility to intrauterine infection of hepatitis B virus.Methods Newborns born by HBsAg positive mothers in Taiyuan infectious hospital were selected and following study were conducted.By simple random sampling,20 newborns with intrauterine infection of HBV or not were selected,respectively.HLA-DR genotyping was conducted with PCR-sequence specific primer (PCR-SSP).The frequency of HLA-DR phenotype and difference between two groups were analyzed.Results The frequency of DR3?DR51 in intrauterine infection group(45%,40%) was higher than in un-infected group(20%,15%).OR was 3.27(0.67-17.08),3.78(0.69-23.02).The frequency of DR53 in un-infected group (60%) was higher than in infected group (35%).OR was 0.36(0.08-1.54).None of differences were significant.Conclusion DR3 and DR51 were the susceptive phenotype and DR53 was the protective phenotype to intrauterine infection of HBV.But further study including more samples was necessary.