水体有机物和蓝灌对BALB／c 3T3细胞的毒性作用

目的：研究水体有机物和蓝藻提取物的毒作用机理。方法：通过形态学观察，MTT法和中性红摄入法，从多个终点检测二者对BALB／c3T3细胞的毒性作用。结果：二者达一定浓度时，可引起细胞形态改变；有机物在各个浓度，各个培养时相均可引起细胞的存活率下降，有明显的剂量－时间－效应关系；而藻毒素在低学短时间染毒下可引起细胞数目的增加，在高浓度或长时间作用下，细胞存活率却明显下降。结论：两种比色法可敏感检测有机物及藻毒素对BALB／c 3T3细胞的毒性作用，两者对细胞膜均有损伤作用；有机物对线粒体有损伤作用，而藻毒素对线粒体具有刺激和损伤的双重作用。

Toxicity of Organism and Cyanobacteria from Water Body on BALB/c Cell

Objective In order to explain the toxic mechanism of organic extract and cyanobacteria extract from water body.Methods The cytotoxicity of these two substances to mouse BALB/c 3T3 cell line in culture was investigated from multi-endpoint. The MTT and neutral red intake microassays and microscopic observation were used respectively.Results The rate of viability decreased, which was dose-time-dependent effect relationship on organic extracts concentration.The cell concentration increased following exposure to cyanobacteria extract at low concentration and short time. In contrary, it decreased at high concentration and long time exposure. Morphological alteration was observed when cell was treated with two substances at comparatively high concentration.Conclusions Toxicity of organic extracts and cyanobacteria extract on 3T3 cell was assessed sensitively by two microassays. Organic extract was harmful on cell membrane and mitochondrion. Cyanobacteria extract can injure the cell membrane, and it had dual role on mitochondrion, one was irritated, and the other was injury.