|
|||||
|
|
| 随机引物PCR方法用于霍乱分子流行病学研究 | |
| 引用本文： | 芮勇宇,蔡初的,萧斌权,俞守义,李建基,廖育煌,王红,钟豪杰,罗不凡.随机引物PCR方法用于霍乱分子流行病学研究[J].中国公共卫生,2000,16(7):630-632. |
| 作者姓名： | 芮勇宇 蔡初的 萧斌权 俞守义 李建基 廖育煌 王红 钟豪杰 罗不凡 |
| 作者单位： | 1. 中国预防医学科学院流行病学微生物研究所,北京,102206 2. 广东省卫生防疫站 3. 广州市卫生防疫站 4. 第一军医大学流行病学教研室,广州,510515 |
| 摘 要： | 研究建立了随机引物ＰＣＲ方法用于霍乱分子浒病学研究，其中两组引物针对霍乱弧菌重复插入序列设计，一组引物为任意序列。对２株霍乱弧菌Ｏ１群古典型（ＣＶＣ）、８１株魂尔托型（ＥＶＣ）和１０株Ｏ１３９群进行了分析，上述菌株经ＰＣＲ鉴定，均携带霍乱肠毒素（ｃｔｘ）基因和毒力协同调节菌毛（ｔｃｐ）基因。３株ＣＶＣ分为２个类型，８１株ＥＶＣ分为１４个类型，１０株Ｏ１３９群分为３个类型。其中１０株分离于一次霍乱暴 |
| 关 键 词： | 霍乱 分子流行病学 随机引物PCR 分型 |
Application of Random Amplified Polymorphic DNA (RAPD) in Molecular Epidemiological Studies of Vibrio Cholerae |
|
| Rui Yongyu.Application of Random Amplified Polymorphic DNA (RAPD) in Molecular Epidemiological Studies of Vibrio Cholerae[J].Chinese Journal of Public Health,2000,16(7):630-632. | |
| Authors: | Rui Yongyu |
| Institution: | Guangzhou 510515 |
| Abstract: | Random amplified polymorphic DNA(RAPD) was established and was applied in molecular epiemiological studies of Vibrio cholerae.Two groups of primer sets were aimed at repetitive sequence of Vibrio cholerae,one group of primer was arbitrary sequence.2 O1 classical(CVC),81 EI Tor and 10 O139 strains were analysed wiht this mehtod.All strains have cholerae toxin gene and toxin coregulated pili gene with the detection of PCR.2 CVC,81 EVC and 10 O139 strains were divided into 2,14 and 3 RAPD types respectively.10 EVC strains isolated from one explosive event were divied into 2 RAPD types.It means that there were several infectious origins in this event.RAPD provides a rapid,simple and discriminable method in molecular epidemiological studies of Vibrio cholerae. |
| Keywords: | Vibrio cholerae\ Random Amplified\ Polymorphic DNA\ Typing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |