2003年广东省流感病毒的流行概况及特征

目的了解2003年广东省流感病毒的流行特点和抗原变异情况。方法根据广东地区流感监测资料进行分析；用交叉血凝抑制试验检测病毒的抗原性；用逆转录-聚合酶链反应扩增病毒HA1基因，纯化产物进行核苷酸序列测定。将序列和Genebank中相关序列进行比较，用MegAlign软件绘制种系发生树。结果全年分离到510株流感病毒，其中H3N2亚型流感481株，占92．4％；乙型流感29株，占7．6％。实验室证实流感爆发52起，其中50起暴发是由H3N2亚型流感病毒引起，2起暴发是由乙型流感病毒引起。H3N2亚型流感病毒抗原性和疫苗侏A／Panama／2007／99（H3N2）有所不同。类似干A／Fujian／411／02（H3N2）。在HAl区氨基酸序列上，和疫苗株A／Panama／2007／99（H3N2）同源性为92．1％，存在有25个氨基酸差异。28株乙型流感病毒属于Victoria系，抗原性类似疫苗侏B／HongKong／330／01，1株乙型病毒属于Yamagata系，抗原性不同干B／sichuan／379／99。结论2003年广东省流感活动比2002年要强；同时流行着甲型（H3N2）和2个谱系的乙型流感病毒，H3N2亚型毒侏是优势侏，抗原性发生了漂移。

Influenza activity and antigenic characterization in Guangdong in 2003

Objective To study influenza activity and antigenic characterization in Guangdong during 2003.Methods The data of influenza surveillance were utilized.Antigenic analysis was performed by using cross hemagglutination inhibition test.The gene of hemagglutinin(HA1)protein were amplified by RT-PCR.PCR products were purified and sequenced.Finally,the sequence data were analyzed with Megalign and Editseq software.Results Of the 510 positive isolates,481(92.4) were in fluenza A(H3N2) viruses,29(7.6%)were influenza B viruses.52 outbreaks were confirmed by lab,including 50 due to influenza A(H3N2)viruses and 2 due to influenza B viruses.Most of influenza A(H3N2)viruses were similar antigenically to A/Fujian/411/02(H3N2),a drift variant of vaccine strain A/Panama/2007/99(H3N2).There were 25 different amino acid on HA1 domain and the homology of amino acid sequence was 92.1% when compared current strains in Guangdong with waccine strain A/Panama/2007/99(H3N2);28 of the influenza B viruses belonged to the B/Victoria lineage and were similar antigenically to the vaccine strain B/HongKong.1 influenza B virus belonged to the B/Yamagata.Conclusion In Guangdong,influenza activity in 2003 was stronger than that in 2002;influenaz A(H3N2)and two lineages of influenza B were co-circulating.The predominant viruses were subtype of influenza(H3N2).And the antigenic drift of influenza(H3N2) occurred.