HBsAg阳性孕妇血清乙肝病毒含量与HBV宫内感染关系的研究

目的探讨孕妇外周血中HBV含量与HBV宫内感染的关系。方法ELISA法检测孕妇及新生儿外周血HBsAg、HBeAg;FQ-PCR对血清HBVDNA定量检测。结果166例新生儿中有28例发生HBV宫内感染(16.87%);在HBV宫内感染组和非感染组孕妇HBVDNA暴露、各级病毒载量与HBV宫内感染趋势χ2检验差异为有统计学意义;HBVDNA?156copies/ml为HBV宫内感染最佳临界预测值,它的敏感度为81.80%,特异度为72.10%,阳性预测值为37.10%,阴性预测值为95.19%。结论孕妇外周血HBVDNA阳性不仅是HBV宫内感染的危险因素,且随着孕妇血中乙肝病毒含量的增高,其发生HBV宫内感染的危险性呈现增高趋势;孕妇血HBVDNA156copies/ml为HBV宫内感染最佳临界预测值,孕妇血清中HBVDNA水平对于预测新生儿HBV宫内感染较为重要。

Study on the relationship between HBV DNA content in HBsAg positive pregnant women and the intrauterine infection

Objective To explore relationship between HBV DNA content in HBsAg positive pregnant women and the HBV intrauterine infection. Methods HBsAg and HBeAg in pregnant woman and their newborns serum were tested by ELISA, HBV DNA were detected by Fluorescence Quantitative PCR (FQ-PCR). Results 28 of 166 newborns were infected by HBV. The value of association between the HBV infection rate and virus content in gravida' serum showed statistical significance. The best critical predictive value of HBV intrauterine infection was 156 copies/ml, the sensitivity, specificity, positive and negative predictive value was 81.80% , 72.10% , 37.10% and 95.19% , respectively. Conclusions The risk of HBV intrauterine transmission showed a increasing trend comparing with the HBV DNA content rising in pregnant women. It was very important to comprehend the HBV DNA level in pregnant women to predict HBV intrauterine infection.