金黄色葡萄球菌肠毒素基因的分型和分布

目的：建立金黄色葡萄球菌肠毒素基因的检测方法。初步研究生牛奶中金黄色葡萄球菌肠毒素基因的分布状况。方法：应用PCR扩增经分离鉴定的金黄色葡萄球菌肠毒素基因SEA-SEJ，电泳检测扩增结果。结果：建立了金黄色葡萄球菌肠毒素基因PCR检测方法。从生牛奶中分离的29株金黄色葡萄球菌检测到SEA-SED和SEG-SEJ基因，没有检测到SEE基因。毒素基因携带率为65．5％，同时携带2种及以上毒素基因的菌株占37．9％。有SEA基因的占51．7％。含其它传统的毒素基因类型SEB、SEC、SED和SEE基因的菌株只占17．2％，携带新发现的毒素基因SEG、SEH、SEI和SEJ的菌株占41．4％。结论：建立的PCR检测金黄色葡萄球菌的肠毒素基因的方法可用于金黄色葡萄球菌肠毒素基因分型和分布的研究。研究表明，生牛奶中分离的金黄色葡萄球菌带毒率高，而且携带新发现的肠毒素基因的较多。

Typing and distribution of toxin genes among Staphylococcus aureus isolates from samples of raw milk

Objective:To develop a typing method for Staphylococcal toxin genes, and to research on the distribution of toxin genes Staphylococcus aureus isolates from raw milk of cows.Methods:Toxin genes were amplified by PCR, and detected by electrophoresis.Results:The typing method for Staphylococccal toxin genes with PCR was developed.The toxin genes of SEA-SED and SEG-SEJ but not SEE could be detected from 29 Staphylococcus aureus isolates from raw milk of cows.Staphylococcus aureus isolates with toxin genes accounted for 65.5% of all the Staphylococcus aureus isolates, the isolates with more than one toxin genes accounted for 37.9%, the isolates with SEA gene accounted for 51.7%, the isolates with other traditional toxin genes only accounted for 17.2%, but the isolates with newly found toxin genes accounted for 41.4%.Conclusion:The developed method for typing Staphylococccal toxin genes with PCR was usableness.Staphylococcus aureus isolates with toxin genes accounted for high ratio, and with newly found toxin genes also accounted for high ratio.