香港海鸥形菌检验技术的建立与应用

目的:建立香港海鸥形菌(Laribacter hongkongensis,LH)的社区获得性胃肠炎患者及淡水鱼类标本采样、运输、培养、分离及鉴定方法。方法:于2005年~2006年,采集社区获得性胃肠炎患者及淡水鱼类新鲜粪样,分别接种SS、XLD、CCDA、TCBS、MacConkey琼脂(MA)以及改良头孢哌酮MacConkey琼脂(CMA)等6种培养基,对典型的菌落,进行常规生化试验,凡氧化酶、触酶试验阳性以及三糖铁试验阴性的菌株,采用特异性PCR检测确诊。结果:社区获得性胃肠炎患者的LH检出率为0.53%(4/759);淡水鱼类草鱼、鲢鱼与鲈鱼的阳性率分别为9.30%、8.33%和5.88%。结论:本文建立的LH表型与基因型诊断技术适合于LH的诊断;当前LH缺乏血清学诊断,发展LH的PCR诊断方法具有现实意义。

Establishment and application of a set of diagnosing methods for Laribacter hongkongensis

Objective:To establish a set of methods for Laribacter hongkongensis sampling,transportation,culture,isolation and identification.Methods:All fecal swabs were freshly collected from outpatients with community-acquired diarrhea and freshwater fish from 2005 to 2006.Samples were inoculated on six culture agar media:XLD,TCBS,SS,CCDA,MacConkey agar(MA) and modified cefoperazone MacConkey agar(CMA).The characteristic colonies on the selective medium were selected out,and tested by three briefness conventional biochemical tests.Those positive on the oxidase and catalase tests,negative on the triple sugar iron agar were selected and identified by special PCR.Results:We discovered four L.hongkongensis strians from 759 outpatients with community-acquired diarrhea,the positive rate was 0.53%.The rate of grass carp,silver carp and perch were 9.30%,8.33 and 5.88%,respectively.Conclusion:A set of diagnosing methods for L.hongkongensis are established.PCR detection is developed to be significant for L.hongkongensis identifying without serological diagnosing methods.