乙型肝炎患者外膜大蛋白与HBV DNA复制的相关性分析及临床意义

目的:探讨血清乙型肝炎病毒外膜大蛋白(HBV-LP)检测对于判定乙型肝炎病毒(HBV)复制的相关性及其临床意义。方法:分别采用ELISA法、时间分辨免疫荧光分析法和实时荧光定量PCR法检测376份乙型肝炎患者血清中HBV-LP、Pre-S1蛋白、HBV-M和HBV DNA,并进行相关性分析。结果:376份乙型肝炎患者血清中HBV-LP水平与HBV DNA拷贝数变化相一致,两者呈正相关(r=0.938,P<0.01);在不同模式的HBeAg血清中HBV-LP与HBV DNA的阳性率差异无统计学意义(P均>0.05);HBV DNA阳性血清中HBV-LP阳性率(92.8%)明显高于Pre-S1蛋白和HBeAg的阳性率(75.1%和41.0%),差异有统计学意义(P均<0.05)。结论:血清HBV-LP水平能反映HBV感染者体内HBV复制程度,其灵敏度高于Pre-S1蛋白和HBeAg,是对HBV M检测的补充,可作为判断HBV复制新的血清学指标。

Large envelope protein of hepatitis B patients with HBV DNA copy of the relevant analy-sis and clinical significance

Objective:To explore the significance of HBV large envelope protein(HBV-LP) in diagnosing HBV replication in chronic hepatitis B patients.Methods:Serum HBV DNA was quantitively detected by using real-time polymerase chain reaction(RT-PCR),The HBV-LP and Pre-S1 were detected by using enzyme linked immunosorbent asay(ELISA) and HBV markers were detected by time differentiate immunofluorescence asay in 376 serum samples collected from chronic hepatitis B patients.Results:Serum HBV-LP level was closely correlated with number of HBV DNA copies(r=0.938,P<0.01).There was no significant difference between positive rate of LHBs and that of HBV DNA in different HBeAg pattern(P>0.05);the positive rate of HBV-LP was 92.8%,which was higher than of that of Pre-S1 and HBeAg which were 75.1% and 41.0%,respectively.There was significant difference(p<0.05=.Conclusion:The level of serum HBV-LP can be used to estimate the state of HBV replication and the sensitivity is superior to both Pre-S1 and HBeAg.It may be used as a new serological marker to detect HBV replication.