| HLA-B27 3种检测方法的比较 |
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| 引用本文： | 朱美玲,罗伟琼,邹翠贤,饶美琼,彭爱军.HLA-B27 3种检测方法的比较[J].中国卫生检验杂志,2007,17(7):1251-1252. |
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| 作者姓名： | 朱美玲 罗伟琼 邹翠贤 饶美琼 彭爱军 |
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| 作者单位： | 1. 广东省深圳市宝安区血站,广东深圳,518101
2. 广东省深圳市宝安区人民医院,广东深圳,518101 |
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| 摘 要： | 目的：比较3种HLA-B27检测方法的敏感度、特异性、适用性。方法：采用微量淋巴细胞毒试验、PCR-SSP和流式细胞仪方法同时检测128份待检标本HLA-B27。结果：淋巴细胞毒试验、PCR-SSP和流式细胞仪方法敏感度、特异性、阳性预测价值、阴性预测价值分别为0.468、0.975、0.917、0.759;1.000、0.988、0.979、1.000;0.978、1.000、1.000、0.988。PCR-SSP和流式细胞仪方法敏感度显著性高于淋巴细胞毒试验,两两比较P〈0.01;阴性预测价值优于淋巴细胞毒试验,两两比较P〈0.05。结论：PCR-SSP和流式细胞仪方法均优于淋巴细胞毒试验,具有较好的临床适用性。
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| 关 键 词： | 微量淋巴细胞毒试验 流式细胞仪 |
| 文章编号： | 1004-8685（2007）07-1251-02 |
| 收稿时间： | 2007-04-20 |
| 修稿时间： | 2007-04-20 |
Comparison of three methods in detecting HLA - B27 |
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| Zhu Mei-ling,Luo Wei-qiong,Zou Cui-xian,Rao Mei-qiong,Peng Ai-jun.Comparison of three methods in detecting HLA - B27[J].Chinses Journal of Health Laboratory Technology,2007,17(7):1251-1252. |
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| Authors: | Zhu Mei-ling Luo Wei-qiong Zou Cui-xian Rao Mei-qiong Peng Ai-jun |
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| Abstract: | Obiective:To evaluate the diagnostic values of three methoods in detecting HLA-B27.Methods:Micro-complement dependent cytotoxicity test,PCR-SSP,flow cytometry were used to detect HLA-B27 in 128 samples.Results:The sensitivity,specificity,predictive value of a positive test,predictive value of a negative test of cytotoxicity test,PCR-SSP,flow cytometry were 0.468,0.975,0.917,0.759;1.000,0.988,0.979,1.000;0.978,1.000,1.000,0.988,respectively.The sensitivities of PCR-SSP and flow cytometry were higher than those of cytotoxicity test,P<0.01.there were obvious differences in the negative predictive values between PCR-SSP and cytotoxicity test,flow cytometry and cytotoxicity test,P<0.05.Conclusion:The diagnostic values of PCR-SSP and flow cytometry in decting HLA-B27 are higher than those of cytotoxicity test.So it can be used in clinical diagnosis. |
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| Keywords: | HLA-B27 PCR-SSP |
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