北京市2004年-2010年志贺菌菌型分布及毒力基因分析

目的:了解近年来北京市志贺菌菌型变化及毒力基因分布特征。方法:对2004年-2010年监测到的1413株志贺菌进行血清分型;应用荧光PCR方法进行ipaHs、et1和sen基因的检测。结果:宋内志贺菌741株(52.4%),福氏志贺菌664株(47.0%),痢疾和鲍氏志贺菌各4株(1.2%)。福氏志贺菌共分为19种血清型,以F4c、F2a、F1a为主。宋内志贺菌自2007年首次超过福氏成为优势菌型后,所占比例逐渐加大,2010年已达到79.3%。福氏志贺菌也逐步由F4c转向以F2a为主,并伴有其它血清型交替出现。ipaH s,et1和sen三种基因的携带率分别为95.4%,82.3%和57.5%。set1和sen在福氏志贺菌的携带率明显高于宋内志贺菌。结论:近8年北京市志贺菌菌型发生了显著变化,宋内志贺菌正逐步成为主导菌型。毒力相关基因在不同菌型的分布存在差异。

Distribution of different serotypes and analysis of virulence genes in Shigella species in Beijing from 2004 to 2010

Objective:To understand the changes of different serotypes of pathogenic bacteria causing the shigellosis and the distribution of virulence gene.Methods:1413 Shigella germ strains isolated from diarrhea patients were examined serotype by serum agglutination.221 strains were detected for ipaH,set1 and sen virulence gene by realtime PCR.Results:741 strains(52.4%) belonged to Shigella Sonnei,664 strains(47%) were Shigella flexneria and other 8 strains(1.2 %) respectively were Shigella dysenteriae and Shigella ...