2004～2006年江西省流感病毒分离情况分析

目的:对2004~2006年江西省流感病毒分离情况分析,以对流感的流行进行预测预报。方法:采集监测医院和疑似流感疫情的流感样病例鼻咽拭子标本,用狗肾传代细胞进行流感病毒分离,采用血凝抑制试验(H I)进行流感病毒型别鉴定。结果:2004~2006年采集咽拭子标本3321份,分离到流感病毒529株,其中H1N1亚型152株,H3N2亚型196株,B型181株;2005年和2006年流感病毒分离率均显著高于2004年(P<0.001);H1N1亚型构成比从0逐年上升;H3N2亚型构成比逐年显著性降低(P<0.001),由优势株变成弱势株;B型由2004年和2005年流行的BY系变为以BV系为主,并在2006年成为优势株;3~4月份、6~7月份为我省流感病毒的活动高峰期,10~11月为低谷期;0~年龄组病毒分离率与60~年龄组病毒分离率无显著性差异,但显著低于其它各年龄组;省级监测医院的总病毒分离率显著高于市级医院的总病毒分离率。结论:2004年江西省流感流行平稳,而2005年和2006年流感病毒活动较活跃;流行优势毒株由H3N2亚型转变为BV型。加强3~4月份及6~7月份流感疫情的监测,加强对市级医院采样的指导。

Analysis on influenza pathogenic surveillance in Jiangxi from 2004 to 2006

Objective:To estimate the prevalence state of influenza by analysis on influenza virus isolated from 2004 to 2006.Methods:The specimens of nasopharyngeal swabs were collected from influenza like cases of hospitals and influenza epidemic situation,and the influenza virus isolated by inoculating the specimens into the MDCK cell.The supernatant of MDCK cell culture were tested by hemagglutination test.All obtained virus strains were identified by hemagglutination inhibition test.Results:A total of 529 strains of influenza virus were isolated from 3321 nasopharyngeal swab specimens collected from 2004 to 2006,152 strains of H1N1 subtype,196 strains of H3N2 subtype and 181 strains of B were identified.The isolation rates of influenza virus in 2005 and in 2006 were all higher than that in 2004(P<0.001).The constituent ratio of H1N1 subtype increased year by year from 0.The constituent ratio of H1N1 subtype obviously decreased year by year(P<0.001),H3N2 became inferior strains from prevailing strains.The prevalence strain of influenza B virus changed to Victoria lineage virus in 2006,which was Yamagata lineage virus in 2004 and 2005,and became the prevailing strains.From March to April and from June to July were the active peak time,from October to November were a low ebb time.The viral isolation rates of aged 0~5 years group and aged 60 years and over group were no significant difference,but significant lower than the other aged groups.The viral isolation rate of the province class surveillance hospitals was significantly higher than that of city class surveillance hospitals.Conclusion:The prevalence of influenza is steady in 2004,but very active in 2005 and 2006.The prevailing strain changed from H3N2 to BV.Monitoring influenza epidemic situation from March to April and from June to July and instructing the nasopharyngeal swab collecting of city class hospitals should be strengthened.