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2000~2004年浙江省食品中产单核李斯特菌污染状况调查
引用本文:梅玲玲,程苏云,朱敏,王赞信.2000~2004年浙江省食品中产单核李斯特菌污染状况调查[J].中国卫生检验杂志,2006,16(7):784-785,815.
作者姓名:梅玲玲  程苏云  朱敏  王赞信
作者单位:浙江省疾病预防控制中心,杭州,310009
摘    要:目的:掌握浙江省食品中产单核李斯特菌污染状况。以及内化素基因(Inl)和李氏溶血素O(Hly)两种致病基因携带情况。方法:按GB4789.30方法分离单增生李斯特菌,采用PCR技术检测李斯特菌的两种致病基因。结果:2000~2004年从2282份食品中共分离产单核李斯特菌163株,总污染率为7.14%(163/2282)。生肉类、熟肉及水产品的污染率分别为12.80%、7.67%和2.24%;114份生食蔬菜中1份标本分离到产单核李斯特菌;285份乳及乳制品、59份冰激凌均未分离到产单核李斯特菌;163株产单核李斯特菌有155株同时检测到内化素基因和李氏溶血素O基因。结论:我省存在发生李斯特菌食物中毒的潜在危险。

关 键 词:浙江省  食品  产单核李斯特菌  内化素  李氏溶血素O
文章编号:1004-8685(2006)07-0784-03
收稿时间:2006-02-05
修稿时间:2006-02-05

Investigation on Listeria monocytogenes contamination in food of Zhejiang Province during 2000 ~ 2004
Mei Ling-ling,Cheng Su-yun,Zhu Ming,Wang Zan-xin.Investigation on Listeria monocytogenes contamination in food of Zhejiang Province during 2000 ~ 2004[J].Chinses Journal of Health Laboratory Technology,2006,16(7):784-785,815.
Authors:Mei Ling-ling  Cheng Su-yun  Zhu Ming  Wang Zan-xin
Institution:Zhejiang Center for Disease Control and Prevention, Hangzhou 310009, China
Abstract:Objective:To find out the practical situation of L.monocytogenes contamination in food of Zhejiang Province and detect carrying rate of the two pathogenic genes Inl and Hly.Methods:L.monocytogenes strain was isolated according to GB-4789.30 and its two pathogenic genes were detected by PCR.Results:An examination on 2 282 samples of food from Zhejiang Province for the detection of L.monocytogenes was carried out in 2000~2004,163 cases of Lm were found,that means a total contamination rate of 7.14%.Its contamination rates detected in raw meat,cooked meat and fishery products were 12.80%,7.67% and 2.24% respectively.L.monocytogenes strain was not detected in the 285 milk or dairy products samples and 59 ice cream samples.Among the total 169 cases,155 of them carried both Listeriolysin O and Internalin gene.Conclusion:There is the potential threat of food poisoning caused by L.monocytogenes in Zhejiang Province.
Keywords:Zhejiang Province  Food  Listeria monocytogenes  Intemalin  Listeriolysin O
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