首页 | 本学科首页   官方微博 | 高级检索  
检索        

经口致敏食物过敏大鼠模型的建立
引用本文:黄娟,钟燕,蔡成,张洪波,唐文静.经口致敏食物过敏大鼠模型的建立[J].卫生研究,2009,38(1).
作者姓名:黄娟  钟燕  蔡成  张洪波  唐文静
作者单位:1. 上海交通大学医学院营养系,上海,200092
2. 上海交通大学医学院附属新华医院临床营养中心
摘    要:目的探讨经口致敏食物过敏大鼠模型的建立方法,以及适宜的评价指标。方法以卵清蛋白(OVA)作为致敏原,将16只3周龄Brown-Norway(BN)大鼠随机分为3组:阴性对照组(生理盐水灌胃)、阳性对照组(OVA腹腔注射组)和实验组(OVA灌胃组),共灌胃9周。在第4、5、6、7、8、9周分别采用双抗体夹心ELISA法及皮肤过敏实验法(PCA)测定实验动物血清OVA特异性IgE(OVA-IgE)抗体滴度,以判断动物是否致敏成功,在第13周各组动物给予100mg/mlOVA1ml灌胃激发后测血清OVA-IgE滴度。结果ELISA结果显示在灌胃第6周时实验组动物OVA-IgE滴度水平达到最高,且显著高于阴性对照组(P<0.05),致敏率为60%(3/5);第7、第8周时OVA-IgE滴度水平略有下降,但是仍然显著高于阴性对照组(P<0.05),致敏率达到80%(4/5),OVA-IgE水平和致敏率与阳性对照组之间差异无显著性;实验组PCA结果在第6、7、8周均显示为阴性,而阳性对照组PCA显示为阳性。结论经口致敏可作为一种可行的建立食物过敏动物模型的方法,符合食物过敏发生的自然生理过程,建议适宜致敏时间为6周;采用ELISA法检测血清OVA-IgE抗体水平较PCA法能更加灵敏地反映经口致敏模型是否成功。

关 键 词:经口致敏  食物过敏  Brown-Norway大鼠  动物模型

An oral sensitization food allergy model in Brown-Norway rats
Abstract:Objective To develop an oral-sensitized animal model of food allergy using Brown-Norway (BN) rats and evaluate the sensitivity of ELISA and passive cutaneous anaphylaxis (PCA) in detecting ovalbumin-specific IgE antibody (OVA-IgE) level in sensitized animals. Methods Sixteen 3-week old female BN rats were randomly divided into 3 groups:negative control group orally gavaged with saline,positive control group sensitized by intraperitoneal injection of 0.1mg/d OVA,and study group sensitized by daily gavage of 1mg/d ovalbumin (OVA). OVA-IgE was analyzed by ELISA and PCA method at week 4,5,6,7,8 and 9. At week 13,OVA-IgE level was analyzed after orally challenged by 1.0ml of 100mg/ml OVA. Results The ELISA result showed that the OVA-IgE level in study group was significantly increased at week 6,7 and week 8 compared with negative control group (P<0.05),and the highest level was found at week 6. There was no significant difference for the level of OVA-IgE between study group and positive control group. The sensitization rate in study group was 60%,80% and 80% at week 6,7 and 8 respectively,which was similar to positive control group. All PCA results in study group were negative,while in positive control group it was positive. Conclusion Oral sensitization could be used as a suitable method to establish an animal model of food allergy,which is more comparable with the natural sensitization process in food allergy patients. ELISA method is more sensitive in detecting OVA-IgE level in oral sensitized animal model than PCA method.
Keywords:Brown-Norway
本文献已被 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号